Mycobacterium phage Plagueis
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Detailed Information for Phage Plagueis
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByIgnacio Ayoroa
Year Found2012
Location FoundSt. Louis, MO US
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.643667 N, 90.2985 W Map
Discovery NotesThe phage was found in parkland, near a waterfall and a pond in a wooded area. The area is near to major walking paths. It is also located near the major road that cuts through the park.
Naming NotesThe word Plagueis sounds similar to plague and although this may sound unoriginal, it is to describe its living conditions, which are very unclean.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 25, 2018
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage137
Sanger Finishing Reactions9
Genome length (bp)41722
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques are very small, with circular shapes. The center of the plaques tend to be a bit cloudy; this is seen throughout most if not all of the plaques. Also, the plaques for the most part are spread out from one another though a few do clump together to form larger areas of plaque.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH450127
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2012WUSLplagueisKH
SEA Lysate Titer 1.66 x 10^10
Date of SEA Lysate Titering Oct 9, 2012
Pitt Freezer Box# 42
Pitt Freezer Box Grid# 26
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload