| Detailed Information for Phage Bakery |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Sharon Kim |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4426 N, 79.9542 W Map |
| Discovery Notes | Soil sample was from Hillman Library. The soil had white streaks in it which could possibly be from fertilizer, and the soil was in presence of small green plants, fallen leaves, mulch, and a large tree. It was 71℉ partly sunny the day I collected the soil. I transported the soil from 11:01am-5:05pm and stored it in a drawer from 5:06am-10:00am the next day before performing the first steps of the direct isolation protocol. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1057 |
| Genome length (bp) | 60468 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DC |
| Subcluster | DC1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | lytic because my plaques were clear |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | size is small; circular; clear |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK937603 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.3x10^9 pfu/mL |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
