Gordonia phage Barb
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Detailed Information for Phage Barb
Discovery Information
Isolation HostGordonia terrae 3612
Duplicates of this PhageMoseph
Found ByOlivia Harlow
Year Found2016
Location FoundGrand Rapids, MI USA
Finding InstitutionCalvin University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.929521 N, 85.588675 W Map
Discovery NotesBarb was found in soil under yellow zinnias in late September. The soil was dark and moist. It was moist likely a combination of regular soil and common potting soil.
Naming NotesThis name was an inside joke between me and my roommate. We both are fans of the show "Stranger Things" in which a character named Barb dies. On the show, her death is brushed off, and, because of that, fans everywhere adopted the saying "Justice for Barb." So, this is Barb's justice.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage220
Genome length (bp)59514
Character of genome endsCircularly Permuted
GC Content67.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDC
SubclusterDC1
Cluster Life CycleTemperate
Lysogeny NotesI believe that Barb is a lytic phage due to the crisp, clear plaques produced and the quickness of those plaques to produce.
Other Cluster Members
Annotating InstitutionGettysburg College
Annotation StatusIn GenBank
Plaque NotesThe plaques changed with the quality of bacteria that was used, and they ranged in size from 2mm-4mm in diameter. The plaques were clear and had crisp edges. Each dilution would result in similar plaques, but it was the different dilutions that would result in different sized plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Barb_1
(helix-turn-helix DNA binding domain protein)

Barb_2

Barb_3

Barb_4

Barb_5
(terminase)

Barb_6

Barb_7

Barb_8
(helix-turn-helix DNA binding domain protein)

Barb_9

Barb_10
(polynucleotide kinase)

Barb_11

Barb_12
(portal protein)

Barb_13

Barb_14
(capsid maturation protease and MuF-like fusion protein)

Barb_15

Barb_16

Barb_17
(DNA binding domain protein)

Barb_18

Barb_19
(RNA ligase)

Barb_20

Barb_21
(major capsid hexamer protein)

Barb_22

Barb_23

Barb_24
(major tail protein)

Barb_25
(head-to-tail adaptor)

Barb_26

Barb_27

Barb_28

Barb_29
(tail assembly chaperone)

Barb_30

Barb_31
(tape measure protein)

Barb_32
(minor tail protein)

Barb_33
(minor tail protein)

Barb_34

Barb_35
(minor tail protein)

Barb_36

Barb_37

Barb_38
(minor tail protein)

Barb_39

Barb_40

Barb_41
(lysin A)

Barb_42

Barb_43

Barb_44

Barb_45

Barb_46
(lysin B)

Barb_47
(integrase)

Barb_48

Barb_49
(integrase)

Barb_50
(immunity repressor)

Barb_51
(helix-turn-helix DNA binding domain protein)

Barb_52

Barb_53

Barb_54

Barb_55

Barb_56
(RecE-like exonuclease)

Barb_57
(RecT-like ssDNA binding protein)

Barb_58

Barb_59

Barb_60

Barb_61
(methyltransferase)

Barb_62
(helix-turn-helix DNA binding domain protein)

Barb_63
(helix-turn-helix DNA binding domain protein)

Barb_64

Barb_65
(RuvC-like resolvase)

Barb_66

Barb_67

Barb_68

Barb_69

Barb_70

Barb_71

Barb_72

Barb_73
(histidine triad nucleotide binding protein)

Barb_74
(nicotinamide riboside transporter)

Barb_75

Barb_76

Barb_77

Barb_78

Barb_79

Barb_80

Barb_81

Barb_82

Barb_83

Barb_84

Barb_85
(DNA methylase)

Barb_86

Barb_87

Barb_88
(DnaJ-like chaperonin)

Barb_89
(helix-turn-helix DNA binding domain protein)

Barb_90

Barb_91

Barb_92

Barb_93

Barb_94

Barb_95

Barb_96

Barb_97

Barb_98

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK864265
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.5x10^8
Pitt Freezer Box# 38
Pitt Freezer Box Grid# H11
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload