Gordonia phage SmokingBunny
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Detailed Information for Phage SmokingBunny
Discovery Information
Isolation HostGordonia terrae 3612
Found ByHyunwoo Kim
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.44558 N, 79.953107 W Map
Discovery NotesThe soil was collected on the sidewalk of Tennyson Avenue at Pittsburgh on the Cathedral of Learning side of the street. It was slightly raining so the soil was little damp and it was very dark. And I saw occasionally people putting fertilizer on that part of the soil.
Naming NotesI hate the smell of cigarette and I also hate the smell of my phage. And I like bunnies.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 15, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage985
Genome length (bp)59454
Character of genome endsCircularly Permuted
GC Content67.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDC
SubclusterDC1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesVery Clear. The capsid is approximately 80nm and the tail is approximately 266.67nm.gm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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SmokingBunny_1
(helix-turn-helix DNA binding domain protein)

SmokingBunny_2

SmokingBunny_3

SmokingBunny_4

SmokingBunny_5
(terminase)

SmokingBunny_6

SmokingBunny_7

SmokingBunny_8
(ASC-1 transcription coactivator)

SmokingBunny_9

SmokingBunny_10
(polynucleotide kinase)

SmokingBunny_11

SmokingBunny_12
(portal protein)

SmokingBunny_13

SmokingBunny_14
(capsid morphogenesis protein)

SmokingBunny_15

SmokingBunny_16
(capsid maturation protease)

SmokingBunny_17
(RNA ligase)

SmokingBunny_18

SmokingBunny_19
(major capsid hexamer protein)

SmokingBunny_20

SmokingBunny_21

SmokingBunny_22
(major tail protein)

SmokingBunny_23
(head-to-tail adaptor)

SmokingBunny_24

SmokingBunny_25

SmokingBunny_26

SmokingBunny_27
(tail assembly chaperone)

SmokingBunny_28
(tail assembly chaperone)

SmokingBunny_29
(tape measure protein)

SmokingBunny_30
(minor tail protein)

SmokingBunny_31
(minor tail protein)

SmokingBunny_32
(minor tail protein)

SmokingBunny_33
(minor tail protein)

SmokingBunny_34

SmokingBunny_35
(minor tail protein)

SmokingBunny_36

SmokingBunny_37

SmokingBunny_38

SmokingBunny_39
(lysin A)

SmokingBunny_40
(holin)

SmokingBunny_41

SmokingBunny_42

SmokingBunny_43

SmokingBunny_44
(lysin B)

SmokingBunny_45
(integrase)

SmokingBunny_46

SmokingBunny_47
(integrase)

SmokingBunny_48
(immunity repressor)

SmokingBunny_49
(excise)

SmokingBunny_50

SmokingBunny_51

SmokingBunny_52

SmokingBunny_53

SmokingBunny_54
(exonuclease)

SmokingBunny_55
(RecT-like ssDNA binding protein)

SmokingBunny_56

SmokingBunny_57

SmokingBunny_58

SmokingBunny_59

SmokingBunny_60
(helix-turn-helix DNA binding domain protein)

SmokingBunny_61

SmokingBunny_62

SmokingBunny_63
(RuvC-like resolvase)

SmokingBunny_64

SmokingBunny_65

SmokingBunny_66

SmokingBunny_67

SmokingBunny_68

SmokingBunny_69

SmokingBunny_70

SmokingBunny_71

SmokingBunny_72

SmokingBunny_73
(nicotinate ribosyltransferase)

SmokingBunny_74

SmokingBunny_75

SmokingBunny_76

SmokingBunny_77

SmokingBunny_78

SmokingBunny_79

SmokingBunny_80

SmokingBunny_81

SmokingBunny_82

SmokingBunny_83
(methyltransferase)

SmokingBunny_84

SmokingBunny_85

SmokingBunny_86
(HNH endonuclease)

SmokingBunny_87
(helix-turn-helix DNA binding domain protein)

SmokingBunny_88

SmokingBunny_89

SmokingBunny_90

SmokingBunny_91

SmokingBunny_92

SmokingBunny_93

SmokingBunny_94

SmokingBunny_95

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK814761
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.18x10^9
Date of SEA Lysate Titering Feb 23, 2017
Pitt Freezer Box# 56
Pitt Freezer Box Grid# D8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload