| Detailed Information for Phage Danyall |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Danyall Saeed |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.441491 N, 79.957521 W Map |
| Discovery Notes | Phage was discovered in a flowerbed after a rain storm as part of a multiple-phage sample, reinforcing the fact that phages thrive in moist environmental conditions. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 566 |
| Genome length (bp) | 58695 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DC |
| Subcluster | DC1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Plaque size did not increase at the rate observed before after steady incubation for about two weeks. This could hint at the phage possibly entering the lysogenic cycle after a certain period of infection and lysis, but this is yet to be determined as a fact. |
| Other Cluster Members |
|
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | Single, isolated plaque (from dilutions) is observed as 3-5 mm in diameter and a central dark spot (1-2 mm in diameter) containing an even darker spot located in about the exact center of the plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH479910 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.67 x 10^10 pfu/mL |
| Date of SEA Lysate Titering |
Oct 28, 2016 |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
