The Actinobacteriophage Database

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Detailed Information for Phage IPhane7
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Dylan Rood
Year Found	2016
Location Found	Waynesville, NC USA
Finding Institution	Western Carolina University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	Unavailable
Discovery Notes	From Compost
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 6, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1739
Genome length (bp)	81036
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	ACCTCCTGCAA
GC Content	61.6%
Fasta file available?	Available upon entering GPS coordinates
Characterization
Cluster	M
Subcluster	M1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Auspice (M1) Bongo (M1) Bricole (M1) Diminimus (M1) Dulcita (M1) FreakyGoo (M1) Glaske16 (M1) IPhane7 (M1) Izel (M1) KleverKiS (M1) LilhomieP (M1) PegLeg (M1) Reindeer (M1) Skinny (M1) SlimJimmy (M1) TyDawg (M1) Aziz (M2) Estes (M2) GardenSalsa (M2) GenevaB15 (M2) MrMagoo (M2) Rey (M2) Nanosmite (M3)
Annotating Institution	Western Carolina University
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	137
Number of tRNAs	19
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View IPhane7_1 IPhane7_2 IPhane7_3 (Lsr2-like DNA bridging protein) IPhane7_4 IPhane7_5 IPhane7_6 IPhane7_7 IPhane7_8 IPhane7_9 IPhane7_10 IPhane7_11 IPhane7_12 IPhane7_13 (terminase small subunit) IPhane7_14 (terminase large subunit) IPhane7_15 (portal protein) IPhane7_16 (MuF-like minor capsid protein) IPhane7_17 (scaffolding protein) IPhane7_18 (major capsid protein) IPhane7_19 (head-to-tail adaptor) IPhane7_20 (head-to-tail stopper) IPhane7_21 IPhane7_22 (tail terminator) IPhane7_23 (major tail protein) IPhane7_24 (tail assembly chaperone) IPhane7_25 (tail assembly chaperone) IPhane7_26 (tape measure protein) IPhane7_27 (minor tail protein) IPhane7_28 (minor tail protein) IPhane7_29 (minor tail protein) IPhane7_30 (minor tail protein) IPhane7_31 (minor tail protein) IPhane7_32 IPhane7_33 IPhane7_34 IPhane7_35 (lysin A) IPhane7_36 IPhane7_37 (holin) IPhane7_38 (lysin B) IPhane7_39 (NrdH-like glutaredoxin) IPhane7_40 IPhane7_41 IPhane7_42 IPhane7_43 IPhane7_44 IPhane7_45 IPhane7_46 IPhane7_47 (nucleotidyl transferase) IPhane7_48 IPhane7_49 IPhane7_50 IPhane7_51 IPhane7_52 IPhane7_53 IPhane7_54 (ERF family ssDNA binding protein) IPhane7_55 IPhane7_56 IPhane7_57 IPhane7_58 (ClpP-like protease) IPhane7_59 IPhane7_60 IPhane7_61 IPhane7_62 IPhane7_63 IPhane7_64 IPhane7_65 IPhane7_66 IPhane7_67 (ssDNA binding protein) IPhane7_68 IPhane7_69 (DnaB-like dsDNA helicase) IPhane7_70 (WhiB family transcription factor) IPhane7_71 (holliday junction resolvase) IPhane7_72 (WhiB family transcription factor) IPhane7_73 IPhane7_74 IPhane7_75 IPhane7_76 IPhane7_77 IPhane7_78 (DnaQ-like DNA polymerase III subunit) IPhane7_79 IPhane7_80 IPhane7_81 (HNH endonuclease) IPhane7_82 (PTPc tyrosine phosphatase) IPhane7_83 IPhane7_84 IPhane7_85 IPhane7_86 IPhane7_87 IPhane7_88 (HNH endonuclease) IPhane7_89 IPhane7_90 — tRNA IPhane7_91 — tRNA IPhane7_92 IPhane7_93 — tRNA IPhane7_94 — tRNA IPhane7_95 — tRNA IPhane7_96 — tRNA IPhane7_97 — tRNA IPhane7_98 — tRNA IPhane7_99 IPhane7_100 — tRNA IPhane7_101 — tRNA IPhane7_102 — tRNA IPhane7_103 IPhane7_104 — tRNA IPhane7_105 (ribonucleotide reductase) IPhane7_106 — tRNA IPhane7_107 — tRNA IPhane7_108 — tRNA IPhane7_109 — tRNA IPhane7_110 — tRNA IPhane7_111 — tRNA IPhane7_112 — tRNA IPhane7_113 (RtcB-like RNA ligase) IPhane7_114 (band-7-like membrane protein) IPhane7_115 (MazG-like nucleotide pyrophosphohydrolase) IPhane7_116 IPhane7_117 IPhane7_118 IPhane7_119 IPhane7_120 IPhane7_121 IPhane7_122 (DprA-like DNA processing chain A) IPhane7_123 IPhane7_124 (metallophosphoesterase) IPhane7_125 IPhane7_126 IPhane7_127 IPhane7_128 IPhane7_129 (serine integrase) IPhane7_130 IPhane7_131 IPhane7_132 IPhane7_133 IPhane7_134 IPhane7_135 IPhane7_136 IPhane7_137 IPhane7_138 IPhane7_139 IPhane7_140 IPhane7_141 IPhane7_142 IPhane7_143 IPhane7_144 IPhane7_145 IPhane7_146 IPhane7_147 IPhane7_148 IPhane7_149 IPhane7_150 IPhane7_151 IPhane7_152 IPhane7_153 IPhane7_154 IPhane7_155 IPhane7_156
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH697587
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	43
Pitt Freezer Box Grid#	F9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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