| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | MyPhage |
| Found By | Tracy Le et al. |
| Year Found | 2011 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.070578 N, 118.453152 W Map |
| Discovery Notes | Isolated in fall 2011 by Team T-9 and Counting (MIMG 103AL): Tracy Le, Jan Michael Taguiam, Jonathan Tran, and Junhao (Jay) Zhang
Instructor (AL): Todd Lorenz
TA (AL): Jovo Vijanderan
Annotated in fall 2012 by Team Pirates of the CURE-ibbeans (MIMG 103BL): Tiffany Chow, Josephine Nguyen, Sean Chang, and Khristina Ipapo
Instructor (BL): Erin Sanders
TA (BL): Will Villella
Curation in winter 2013 by (MIMG 199): Tiffany Chow, Khristina Ipapo, and Shannon Cooper
|
| Naming Notes | The new phage name was given by Tiffany Chow, Khristina Ipapo, and Shannon Cooper who curated the genome annotation results during winter quarter 2013. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 14, 2013 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 150 |
| Genome length (bp) | 80955 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Plaque morphology indicates a lytic life cycle. However, the presence of an integrase gene is an area of interest. Further investigation using bioinformatics indicates that this is a remnant integrase, indicating that it could have recently evolved from a temperate phage. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are round and clear with an average diameter of 2mm. The size range is 1mm to 3mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 138 |
| Number of tRNAs | 20 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KC900379 |
| Refseq Number | NC_021299 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011UCLAmspb01JT |
| SEA Lysate Titer |
6.4x10^9 |
| Date of SEA Lysate Titering |
Oct 27, 2011 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
E9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
