Gordonia phage Perkunas
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Detailed Information for Phage Perkunas
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Former namesAbaddon
Found ByGrace Murley
Year Found2023
Location FoundTemple Terrace, FL United States
Finding InstitutionUniversity of South Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature32°C
GPS Coordinates28.023 N, 82.4 W Map
Discovery NotesConditions upon discovery: 32 degrees Celsius, damp/wet mushy soil that received little to no light, humidity at 60 %, time of collection at 2:19 PM

This phage was located beside a storm drain and the combination of lack of sunlight and the recent rainstorm had left the soil extremely damp.
Naming NotesPerkūnas is the name of the Lithuanian God of thunder and storms. Being that I found this phage by a storm drain, I found this name fitting. The Lithuanian origin was chosen because I'm 25% Lithuanian.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 6, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1358
Genome length (bp)59575
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCTCT
GC Content51.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDJ
Subcluster--
Cluster Life CycleLytic
Lysogeny Notesinfected Gordonia Rubripertincta (host), Gordonia Terrae, and Gordonia Westfalica
Other Cluster Members
Annotating InstitutionUniversity of South Florida
Annotation StatusIn GenBank
Plaque NotesPlaques are clear and exist in large and small plaques, mainly large.
MorphotypeSiphoviridae
Number of Genes94
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Perkunas_1

Perkunas_2

Perkunas_3

Perkunas_4

Perkunas_5

Perkunas_6

Perkunas_7

Perkunas_8

Perkunas_9

Perkunas_10
(hydrolase)

Perkunas_11
(lysin A)

Perkunas_12
(holin)

Perkunas_13

Perkunas_14
(hydrolase)

Perkunas_15
(terminase)

Perkunas_16

Perkunas_17

Perkunas_18

Perkunas_19
(portal protein)

Perkunas_20

Perkunas_21
(major capsid protease fusion protein)

Perkunas_22

Perkunas_23
(tail terminator)

Perkunas_24
(major tail protein)

Perkunas_25

Perkunas_26
(major tail protein)

Perkunas_27

Perkunas_28

Perkunas_29
(lysin B)

Perkunas_30

Perkunas_31
(tape measure protein)

Perkunas_32
(minor tail protein)

Perkunas_33
(minor tail protein)

Perkunas_34

Perkunas_35

Perkunas_36

Perkunas_37

Perkunas_38

Perkunas_39

Perkunas_40

Perkunas_41

Perkunas_42

Perkunas_43

Perkunas_44

Perkunas_45

Perkunas_46

Perkunas_47

Perkunas_48

Perkunas_49

Perkunas_50

Perkunas_51

Perkunas_52

Perkunas_53

Perkunas_54

Perkunas_55

Perkunas_56

Perkunas_57

Perkunas_58

Perkunas_59

Perkunas_60

Perkunas_61

Perkunas_62

Perkunas_63

Perkunas_64
(DNA primase/polymerase)

Perkunas_65

Perkunas_66

Perkunas_67
(deoxycytidylate deaminase)

Perkunas_68

Perkunas_69

Perkunas_70
(SSB protein)

Perkunas_71

Perkunas_72

Perkunas_73
(ThyX-like thymidylate synthase)

Perkunas_74

Perkunas_75

Perkunas_76

Perkunas_77

Perkunas_78

Perkunas_79

Perkunas_80

Perkunas_81

Perkunas_82

Perkunas_83

Perkunas_84

Perkunas_85

Perkunas_86

Perkunas_87

Perkunas_88
(DNA helicase)

Perkunas_89

Perkunas_90
(HNH endonuclease)

Perkunas_91
(VRR-Nuc domain protein)

Perkunas_92
(Cas4 exonuclease)

Perkunas_93

Perkunas_94

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978885
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 11000000000 PFU/ml
Pitt Freezer Box# 172
Pitt Freezer Box Grid# G10
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload