| Detailed Information for Phage Perkunas |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Former names | Abaddon |
| Found By | Grace Murley |
| Year Found | 2023 |
| Location Found | Temple Terrace, FL United States |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 32°C |
| GPS Coordinates | 28.023 N, 82.4 W Map |
| Discovery Notes | Conditions upon discovery: 32 degrees Celsius, damp/wet mushy soil that received little to no light, humidity at 60 %, time of collection at 2:19 PM
This phage was located beside a storm drain and the combination of lack of sunlight and the recent rainstorm had left the soil extremely damp. |
| Naming Notes | Perkūnas is the name of the Lithuanian God of thunder and storms. Being that I found this phage by a storm drain, I found this name fitting. The Lithuanian origin was chosen because I'm 25% Lithuanian. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1358 |
| Genome length (bp) | 59575 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | infected Gordonia Rubripertincta (host), Gordonia Terrae, and Gordonia Westfalica |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and exist in large and small plaques, mainly large. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978885 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
11000000000 PFU/ml |
| Pitt Freezer Box# |
172 |
| Pitt Freezer Box Grid# |
G10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
