| Detailed Information for Phage PestoPenguin |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Andrea DeLucca |
| Year Found | 2024 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.446292 N, 79.952433 W Map |
| Discovery Notes | Andrea DeLucca, Kaitlyn Limbaugh, Cara Munksgard
The sample was collected from a bed where there were bushes, right alongside a parking lot and right next to a high traffic sidewalk. The soil was quite dry and there was mulch in the bed that was covering the soil and needed to be moved in order to obtain the sample. |
| Naming Notes | The name is a reference to the penguin at the Melbourne Zoo, Pesto. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 6, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 4580 |
| Genome length (bp) | Unknown |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 52.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | Being Annotated (Expected completion by 12/31/2025) |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
214 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Plaque Picture | Download |
