| Detailed Information for Phage Rey |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Christina Ferreira |
| Year Found | 2008 |
| Location Found | Houston (Harris County), TX USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.725031 N, 95.28519 W Map |
| Growth Notes | In order to recover Rey plaques and make a lysate, it is helpful to use only 250 ul of M. smegmatis culture to make the lawn and incubate plates at room temperature over a few days. (Greg Broussard, 2013.) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 11, 2011 |
| Genome length (bp) | 83724 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCCCATGCAA |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 153 |
| Number of tRNAs | 21 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | JF937105 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
H1 |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |