Mycobacterium phage Skinny
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| Detailed Information for Phage Skinny | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jenna Curran, Kristen Rose |
| Year Found | 2021 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.467601 N, 94.723749 W Map |
| Discovery Notes | We collected a sample of soil right outside of Speer Chapel on the campus of LeTourneau University. It was cloudy and humid when the sample was collected. It had been raining the night before, so the soil was fairly moist. There were lots of thin roots in the soil, and ants were seen in the surrounding soil. There were also small holes, possibly for snakes or worms, in the surrounding soil. |
| Naming Notes | One day in lab, we were doing serial dilutions for a round of purification. Dr. Baliraine asked us how we told our smeg cell tubes and our phage buffer tubes apart. We said the phage buffer tubes (with our plaque) had the "skinny" sharpie marker on them. And from there we've called our phage "Skinny." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 1, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 499 |
| Genome length (bp) | 82071 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 141 |
| Number of tRNAs | 18 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP297551 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 134 |
| Pitt Freezer Box Grid# | D9 |
| Available Files | |
| GenBank File for Phamerator | Download |