| Detailed Information for Phage SmokingBunny |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Hyunwoo Kim |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.44558 N, 79.953107 W Map |
| Discovery Notes | The soil was collected on the sidewalk of Tennyson Avenue at Pittsburgh on the Cathedral of Learning side of the street. It was slightly raining so the soil was little damp and it was very dark. And I saw occasionally people putting fertilizer on that part of the soil. |
| Naming Notes | I hate the smell of cigarette and I also hate the smell of my phage. And I like bunnies. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 15, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 985 |
| Genome length (bp) | 59454 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DC |
| Subcluster | DC1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Very Clear. The capsid is approximately 80nm and the tail is approximately 266.67nm.gm |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK814761 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.18x10^9 |
| Date of SEA Lysate Titering |
Feb 23, 2017 |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
