| Detailed Information for Phage Barb | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Duplicates of this Phage | Moseph |
| Found By | Olivia Harlow |
| Year Found | 2016 |
| Location Found | Grand Rapids, MI USA |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.929521 N, 85.588675 W Map |
| Discovery Notes | Barb was found in soil under yellow zinnias in late September. The soil was dark and moist. It was moist likely a combination of regular soil and common potting soil. |
| Naming Notes | This name was an inside joke between me and my roommate. We both are fans of the show "Stranger Things" in which a character named Barb dies. On the show, her death is brushed off, and, because of that, fans everywhere adopted the saying "Justice for Barb." So, this is Barb's justice. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 220 |
| Genome length (bp) | 59514 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DC |
| Subcluster | DC1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | I believe that Barb is a lytic phage due to the crisp, clear plaques produced and the quickness of those plaques to produce. |
| Other Cluster Members |
Click to ViewArri (DC1) Bakery (DC1) Barb (DC1) CherryCola (DC1) ClamChowder (DC1) CoveyB (DC1) Danyall (DC1) Egi03 (DC1) Evamon (DC1) Fireball (DC1) Fugax (DC1) Gezellig (DC1) Halo3 (DC1) Jambalaya (DC1) KimmyK (DC1) Mutzi (DC1) Nubi (DC1) Phlop (DC1) PinkCoffee (DC1) Portcullis (DC1) PullumCavea (DC1) RogerDodger (DC1) Salvador (DC1) Savbucketdawg (DC1) Shinji (DC1) SmokingBunny (DC1) TillyBobJoe (DC1) Togo (DC1) Twister6 (DC1) Valary (DC1) VanDeWege (DC1) Wizard (DC1) YungMoney (DC1) Clown (DC2) |
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques changed with the quality of bacteria that was used, and they ranged in size from 2mm-4mm in diameter. The plaques were clear and had crisp edges. Each dilution would result in similar plaques, but it was the different dilutions that would result in different sized plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBarb_1 Barb_2 Barb_3 Barb_4 Barb_5 Barb_6 Barb_7 Barb_8 Barb_9 Barb_10 Barb_11 Barb_12 Barb_13 Barb_14 Barb_15 Barb_16 Barb_17 Barb_18 Barb_19 Barb_20 Barb_21 Barb_22 Barb_23 Barb_24 Barb_25 Barb_26 Barb_27 Barb_28 Barb_29 Barb_30 Barb_31 Barb_32 Barb_33 Barb_34 Barb_35 Barb_36 Barb_37 Barb_38 Barb_39 Barb_40 Barb_41 Barb_42 Barb_43 Barb_44 Barb_45 Barb_46 Barb_47 Barb_48 Barb_49 Barb_50 Barb_51 Barb_52 Barb_53 Barb_54 Barb_55 Barb_56 Barb_57 Barb_58 Barb_59 Barb_60 Barb_61 Barb_62 Barb_63 Barb_64 Barb_65 Barb_66 Barb_67 Barb_68 Barb_69 Barb_70 Barb_71 Barb_72 Barb_73 Barb_74 Barb_75 Barb_76 Barb_77 Barb_78 Barb_79 Barb_80 Barb_81 Barb_82 Barb_83 Barb_84 Barb_85 Barb_86 Barb_87 Barb_88 Barb_89 Barb_90 Barb_91 Barb_92 Barb_93 Barb_94 Barb_95 Barb_96 Barb_97 Barb_98 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK864265 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.5x10^8 |
| Pitt Freezer Box# | 38 |
| Pitt Freezer Box Grid# | H11 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |