| Detailed Information for Phage Barb |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Duplicates of this Phage | Moseph |
| Found By | Olivia Harlow |
| Year Found | 2016 |
| Location Found | Grand Rapids, MI USA |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.929521 N, 85.588675 W Map |
| Discovery Notes | Barb was found in soil under yellow zinnias in late September. The soil was dark and moist. It was moist likely a combination of regular soil and common potting soil. |
| Naming Notes | This name was an inside joke between me and my roommate. We both are fans of the show "Stranger Things" in which a character named Barb dies. On the show, her death is brushed off, and, because of that, fans everywhere adopted the saying "Justice for Barb." So, this is Barb's justice. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 220 |
| Genome length (bp) | 59514 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DC |
| Subcluster | DC1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | I believe that Barb is a lytic phage due to the crisp, clear plaques produced and the quickness of those plaques to produce. |
| Other Cluster Members |
|
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques changed with the quality of bacteria that was used, and they ranged in size from 2mm-4mm in diameter. The plaques were clear and had crisp edges. Each dilution would result in similar plaques, but it was the different dilutions that would result in different sized plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK864265 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.5x10^8 |
| Pitt Freezer Box# |
38 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
