| Detailed Information for Phage FuzzBuster |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Brooklynn Herold |
| Year Found | 2018 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.3025 N, 83.183333 W Map |
| Discovery Notes | Creek bed soil from Cullowhee Creek, adjacent to Ramsey center, at the second (older) wooden bridge. Soil was in a well shaded area due to a close by tree, and out of direct exposure from people in consideration of the location on campus. |
| Naming Notes | Due to the fuzzy or cloudy plaques that this specific phage created, I thought it was appropriate to include fuzz into the name. The ending portion of the name was determined by my experience in trying to the phage to grow because it almost disappeared, but I was able to revive the virus. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 751 |
| Genome length (bp) | 54844 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | Concistently small specks that vary from clear to cloudy; turbid plaques appear more popular. |
| Morphotype | Siphoviridae |
| Number of Genes | 85 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN062720 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4x10^10 PFU/mL |
| Pitt Freezer Box# |
81 |
| Pitt Freezer Box Grid# |
C4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
