| Detailed Information for Phage GardenSalsa |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sam McKee |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | Seton Hill University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.337159 N, 80.081037 W Map |
| Discovery Notes | This phage was isolated from a soil sample obtained from a compost, mulch pile near a vegetable garden on 8/29/2015 at 18:00. The air temperature was 82°F. The sample was taken about 1 foot into the pile at about 3 inches in depth. The soil was light brown in color and appeared very dry. There were signs of biological life in the area and there was surrounding vegetation like decomposing grass and vegetables. The sample site was approximately 6 feet from the base of a maple tree. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 343 |
| Genome length (bp) | 80309 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCCCATGCAA |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque appeared to be turbid and cloudy and is about 0.5 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 142 |
| Number of tRNAs | 22 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY783914 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
21 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
