| Detailed Information for Phage Gilgamesh |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Michael Almisry, Madeline Lee |
| Year Found | 2015 |
| Location Found | St Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 38.645 N, 90.311 W Map |
| Discovery Notes | Collected a few feet from a brick gate and pedestrian walkway on the Washington University in St. Louis campus. |
| Naming Notes | Gilgamesh is a legendary Sumerian king and the protagonist of what is often cited as the first great literary work, the Epic of Gilgamesh. He's pretty cool. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 3, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 136 |
| Genome length (bp) | 129136 |
| Character of genome ends | Circularly Permuted |
| End Determination Notes | No obvious read buildups in either direction, nor any apparent bumps in coverage. |
| GC Content | 71.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Very small plaque size (0.5-0.75 mm in diameter), circular shape, hazy border |
| Morphotype | Siphoviridae |
| Number of Genes | 156 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN234216 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Designator |
Gilgamesh |
| SEA Lysate Titer |
1.3 x 10^9 |
| Date of SEA Lysate Titering |
Oct 1, 2015 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
