| Detailed Information for Phage Hughesyang |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tyler Hughes and Jeier Yang |
| Year Found | 2014 |
| Location Found | West Lafayette, IN USA |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.4259 N, 86.9081 W Map |
| Discovery Notes | Found in moist soil the day after a fairly heavy rain. The soil that the phage was isolated from was roughly 10-12cm below the surface. |
| Naming Notes | The name of the phage is just the last names of the two people who discovered the phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 296 |
| Genome length (bp) | 112884 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.9% |
| Sequencing Notes | There were two deletions present in this phage population, each one in about 50% of the population. Deletion 1 is 2695 bp long, and is between positions 35179 - 37873, inclusive. Deletion 2 is 2464 bp and is between positions 49879 - 52342, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are typically between 2.5 and 3mm in diameter and have completely clear centers with fuzzy edges. They are uniformly circular. |
| Morphotype | Siphoviridae |
| Number of Genes | 241 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524504 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.72e8 pfu/mL |
| Pitt Freezer Box# |
12 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
