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Ixel (EA11) Nebulous (EA5) Gandalph (F1) Coco12 (F1) Rebel (N)
Mycobacterium phage JuicyJay
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Detailed Information for Phage JuicyJay
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEmmett Askira
Year Found2015
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates41.828991 N, 71.399643 W Map
Discovery NotesSoil originated from a planter on the Southeast corner of Brook and Meeting streets that contained a mixture of soil and mulch, with some roots. The sample was drawn from the surface to about 3cm of depth. The temperature at the time of collection was 21C. JuicyJay was found using direct plating.
Naming NotesThe phage is predicted to be of cluster J. A musical artist has a similar name, but spelled differently, and declined the invitation to have phage named after him.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1007
Genome length (bp)113075
Character of genome ends3' Sticky Overhang
Overhang Length4 bases
Overhang SequenceATCC
GC Content60.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterJ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWebster University
Annotation StatusBeing Annotated (Expected completion by 2/5/2020)
Plaque NotesPlaques are cloudy, and of widely variable size. In some, the cloudiness is evenly distributed in a ring, while in others there is a tail, resembling a short comet's tail of cloudiness in one direction, while the other edge is fairly defined. Plaques range from less than 0.5mm diameter to a maximum around 4mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.1 x 10^10
Date of SEA Lysate Titering Oct 23, 2015
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload