The Actinobacteriophage Database

Mycobacterium phage JuicyJay

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Detailed Information for Phage JuicyJay
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Emmett Askira
Year Found	2015
Location Found	Providence, RI USA
Finding Institution	Brown University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	Not entered
GPS Coordinates	41.828991 N, 71.399643 W Map
Discovery Notes	Soil originated from a planter on the Southeast corner of Brook and Meeting streets that contained a mixture of soil and mulch, with some roots. The sample was drawn from the surface to about 3cm of depth. The temperature at the time of collection was 21C. JuicyJay was found using direct plating.
Naming Notes	The phage is predicted to be of cluster J. A musical artist has a similar name, but spelled differently, and declined the invitation to have phage named after him.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 15, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	1007
Genome length (bp)	113075
Character of genome ends	3' Sticky Overhang
Overhang Length	4 bases
Overhang Sequence	ATCC
GC Content	60.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	J
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Ariel Bagrid BAKA Beem Bobby Bombitas Constella Courthouse Dallas DmpstrDiver Dove Duke13 Ejimix EricMillard Gonephishing Halley Hannaconda HokkenD Hughesyang JuicyJay Kalah2 KashFlow Klein LittleE Lucky2013 MiaZeal Minerva NihilNomen Odette Omega Optimus Phoebus Porcelain Pound Redno2 Schatzie Squint Superphikiman Thibault ThreeRngTarjay Wanda Yeet Zelink
Annotating Institution	Webster University
Annotation Status	In GenBank
Plaque Notes	Plaques are cloudy, and of widely variable size. In some, the cloudiness is evenly distributed in a ring, while in others there is a tail, resembling a short comet's tail of cloudiness in one direction, while the other edge is fairly defined. Plaques range from less than 0.5mm diameter to a maximum around 4mm.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View JuicyJay_1 (HNH endonuclease) JuicyJay_2 (terminase small subunit) JuicyJay_3 JuicyJay_4 JuicyJay_5 (HNH endonuclease) JuicyJay_6 JuicyJay_7 JuicyJay_8 JuicyJay_9 JuicyJay_10 JuicyJay_11 (terminase large subunit) JuicyJay_12 JuicyJay_13 (portal protein) JuicyJay_14 (capsid maturation protease) JuicyJay_15 (major capsid protein) JuicyJay_16 (glycosyltransferase) JuicyJay_17 (serine/threonine kinase) JuicyJay_18 (methyltransferase) JuicyJay_19 (glycosyltransferase) JuicyJay_20 (glycosyltransferase) JuicyJay_21 (glycosyltransferase) JuicyJay_22 (protease) JuicyJay_23 (UDP-glucose dehydrogenase) JuicyJay_24 JuicyJay_25 (glycosyltransferase) JuicyJay_26 JuicyJay_27 (head-to-tail adaptor) JuicyJay_28 (head-to-tail stopper) JuicyJay_29 JuicyJay_30 (tail terminator) JuicyJay_31 JuicyJay_32 (major tail protein) JuicyJay_33 (tail assembly chaperone) JuicyJay_34 (tail assembly chaperone) JuicyJay_35 (tape measure protein) JuicyJay_36 (endonuclease VII) JuicyJay_37 (minor tail protein) JuicyJay_38 (minor tail protein) JuicyJay_39 (minor tail protein) JuicyJay_40 (minor tail protein) JuicyJay_41 (minor tail protein) JuicyJay_42 (minor tail protein) JuicyJay_43 JuicyJay_44 JuicyJay_45 (minor tail protein) JuicyJay_46 JuicyJay_47 JuicyJay_48 JuicyJay_49 (lysin A) JuicyJay_50 JuicyJay_51 (lysin B) JuicyJay_52 JuicyJay_53 JuicyJay_54 (thioredoxin) JuicyJay_55 JuicyJay_56 JuicyJay_57 JuicyJay_58 (Lsr2-like DNA bridging protein) JuicyJay_59 JuicyJay_60 JuicyJay_61 JuicyJay_62 JuicyJay_63 JuicyJay_64 JuicyJay_65 JuicyJay_66 JuicyJay_67 JuicyJay_68 JuicyJay_69 JuicyJay_70 JuicyJay_71 JuicyJay_72 JuicyJay_73 JuicyJay_74 JuicyJay_75 JuicyJay_76 JuicyJay_77 JuicyJay_78 (excise) JuicyJay_79 (tyrosine integrase) JuicyJay_80 JuicyJay_81 JuicyJay_82 JuicyJay_83 JuicyJay_84 JuicyJay_85 JuicyJay_86 JuicyJay_87 JuicyJay_88 JuicyJay_89 JuicyJay_90 JuicyJay_91 JuicyJay_92 JuicyJay_93 (immunity repressor) JuicyJay_94 (helix-turn-helix DNA binding domain protein) JuicyJay_95 JuicyJay_96 JuicyJay_97 (esterase) JuicyJay_98 JuicyJay_99 JuicyJay_100 JuicyJay_101 JuicyJay_102 JuicyJay_103 (HNH endonuclease) JuicyJay_104 JuicyJay_105 JuicyJay_106 JuicyJay_107 JuicyJay_108 JuicyJay_109 JuicyJay_110 JuicyJay_111 JuicyJay_112 JuicyJay_113 JuicyJay_114 (RecB-like exonuclease) JuicyJay_115 JuicyJay_116 JuicyJay_117 JuicyJay_118 (methyltransferase) JuicyJay_119 (methyltransferase) JuicyJay_120 (HNH endonuclease) JuicyJay_121 JuicyJay_122 JuicyJay_123 JuicyJay_124 JuicyJay_125 (polynucleotide kinase) JuicyJay_126 JuicyJay_127 (endonuclease VII) JuicyJay_128 (WhiB family transcription factor) JuicyJay_129 (helix-turn-helix DNA binding domain protein) JuicyJay_130 JuicyJay_131 JuicyJay_132 JuicyJay_133 (DNA primase/polymerase) JuicyJay_134 JuicyJay_135 JuicyJay_136 JuicyJay_137 JuicyJay_138 JuicyJay_139 JuicyJay_140 (helix-turn-helix DNA binding domain protein) JuicyJay_141 JuicyJay_142 JuicyJay_143 JuicyJay_144 (DNA primase/helicase) JuicyJay_145 JuicyJay_146 JuicyJay_147 JuicyJay_148 (HNH endonuclease) JuicyJay_149 JuicyJay_150 JuicyJay_151 JuicyJay_152 (RNA ligase) JuicyJay_153 (ClpP-like protease) JuicyJay_154 (DnaQ-like DNA polymerase III subunit) JuicyJay_155 JuicyJay_156 (HNH endonuclease) JuicyJay_157 JuicyJay_158 JuicyJay_159 (nucleotide pyrophosphohydrolase) JuicyJay_160 JuicyJay_161 JuicyJay_162 (MRE11 double-strand break endo/exonuclease) JuicyJay_163 JuicyJay_164 JuicyJay_165 JuicyJay_166 JuicyJay_167 JuicyJay_168 JuicyJay_169 JuicyJay_170 (metallophosphoesterase) JuicyJay_171 JuicyJay_172 JuicyJay_173 JuicyJay_174 JuicyJay_175 JuicyJay_176 JuicyJay_177 JuicyJay_179 JuicyJay_180 JuicyJay_181 JuicyJay_182 JuicyJay_183 JuicyJay_184 JuicyJay_185 JuicyJay_186 JuicyJay_187 JuicyJay_188 JuicyJay_189 JuicyJay_190 (immunity repressor) JuicyJay_191 JuicyJay_192 JuicyJay_193 (FtsK-like DNA translocase) JuicyJay_194 JuicyJay_195 JuicyJay_196 JuicyJay_197 (Ku-like dsDNA break-binding protein) JuicyJay_198 JuicyJay_199 JuicyJay_200 JuicyJay_201 JuicyJay_202 JuicyJay_203 JuicyJay_204 (helix-turn-helix DNA binding domain protein) JuicyJay_205 JuicyJay_206 JuicyJay_207 JuicyJay_208 JuicyJay_209 JuicyJay_210 JuicyJay_211 JuicyJay_212 JuicyJay_213 JuicyJay_214 JuicyJay_215 JuicyJay_216 JuicyJay_217 JuicyJay_218 JuicyJay_219 JuicyJay_220 JuicyJay_221 JuicyJay_222 JuicyJay_223 JuicyJay_224 JuicyJay_225 JuicyJay_226 JuicyJay_227 JuicyJay_228 JuicyJay_229 JuicyJay_230 JuicyJay_231 JuicyJay_232 JuicyJay_233 JuicyJay_234 JuicyJay_235 JuicyJay_236
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MZ958748
Refseq Number	None yet
Archiving Info
Archiving status	Not in Pitt Archives
SEA Lysate Titer	2.1 x 10^10
Date of SEA Lysate Titering	Oct 23, 2015
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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