Mycobacterium phage Wanda
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Detailed Information for Phage Wanda
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJames Bowen
Year Found2011
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.604722 N, 75.375277 W Map
Discovery NotesSoil was from a large sink hole opened up from Hurricane Irene. Depth about 2 feet. Soil was neither very moist nor very dry.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 24, 2012
Sequencing FacilityDavid H. Murdock Research Institute
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage252
Sanger Finishing Reactions2
Genome length (bp)109960
Character of genome ends3' Sticky Overhang
Overhang Length4 bases
Overhang SequenceATCC
GC Content60.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterJ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes"Bulls Eye". Very small (1-2 mm) after 24 hours. After 48 hours plaques usually develop Halo-effect with turbid-edges. After 48 hours of incubation plaques are between 4-5 mm. If plaques are very crowded then bulls-eyes seem to develop at 48 hours too.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Wanda_1
(HNH endonuclease)

Wanda_2
(terminase small subunit)

Wanda_3
(enoyl-CoA-hydratase)

Wanda_4

Wanda_5

Wanda_6

Wanda_7

Wanda_8

Wanda_9

Wanda_10
(terminase large subunit)

Wanda_11

Wanda_12
(portal protein)

Wanda_13
(capsid maturation protease)

Wanda_14
(major capsid protein)

Wanda_15
(glycosyl transferase)

Wanda_16
(ser/thr kinase)

Wanda_17
(methyl transferase)

Wanda_18
(glycosyl transferase)

Wanda_19
(glycosyl transferase)

Wanda_20
(glycosyl transferase)

Wanda_21
(Zn-finger protease)

Wanda_22

Wanda_23

Wanda_24
(glycosyl transferase)

Wanda_25

Wanda_26
(head-to-tail adaptor)

Wanda_27
(head-to-tail stopper)

Wanda_28

Wanda_29
(tail terminator)

Wanda_30

Wanda_31

Wanda_32
(major tail subunit)

Wanda_33
(tail assembly chaperone)

Wanda_34
(tail assembly chaperone)

Wanda_35
(tapemeasure)

Wanda_36
(minor tail protein)

Wanda_37
(minor tail protein)

Wanda_38
(minor tail protein)

Wanda_39
(D-ala-D-ala carboxypeptidase)

Wanda_40

Wanda_41

Wanda_42

Wanda_43

Wanda_44

Wanda_45

Wanda_46

Wanda_47

Wanda_48

Wanda_49

Wanda_50

Wanda_51

Wanda_52
(HNH endonuclease)

Wanda_53
(LysA)

Wanda_54

Wanda_55
(LysB)

Wanda_56
(holin)

Wanda_57

Wanda_58
(NrdH-like protein)

Wanda_59

Wanda_60

Wanda_61

Wanda_62

Wanda_63

Wanda_64

Wanda_65
(Lsr2)

Wanda_66

Wanda_67

Wanda_68

Wanda_69

Wanda_70

Wanda_71

Wanda_72

Wanda_73

Wanda_74

Wanda_75

Wanda_76

Wanda_77

Wanda_78

Wanda_79

Wanda_80

Wanda_81

Wanda_82

Wanda_83

Wanda_84
(Xis)

Wanda_85
(integrase)

Wanda_86

Wanda_87

Wanda_88

Wanda_89

Wanda_90

Wanda_91

Wanda_92

Wanda_93

Wanda_94

Wanda_95

Wanda_96

Wanda_97

Wanda_98

Wanda_99

Wanda_100
(immunity repressor)

Wanda_101

Wanda_102

Wanda_103
(LipC)

Wanda_104

Wanda_105

Wanda_106

Wanda_107

Wanda_108

Wanda_109

Wanda_110

Wanda_111

Wanda_112

Wanda_113

Wanda_114

Wanda_115

Wanda_116

Wanda_117

Wanda_118
(AddA-like protein)

Wanda_119

Wanda_120

Wanda_121
(methylase)

Wanda_122
(HNH endonuclease)

Wanda_123

Wanda_124

Wanda_125

Wanda_126

Wanda_127
(polynucleotide kinase)

Wanda_128

Wanda_129
(Endo VII)

Wanda_130
(WhiB)

Wanda_131
(HTH DNA binding domain protein)

Wanda_132

Wanda_133

Wanda_134

Wanda_135

Wanda_136
(DNA polymerase/primase)

Wanda_137
(HNH endonuclease)

Wanda_138

Wanda_139

Wanda_140

Wanda_141

Wanda_142
(HTH DNA binding domain protein)

Wanda_143

Wanda_144
(HTH DNA binding domain protein)

Wanda_145

Wanda_146

Wanda_147
(DnaB-like helicase)

Wanda_148
(HNH endonuclease)

Wanda_149

Wanda_150

Wanda_151

Wanda_152

Wanda_153
(HNH endonuclease)

Wanda_154

Wanda_155

Wanda_156
(RNA ligase)

Wanda_157
(ClpP)

Wanda_158
(DnaQ)

Wanda_159

Wanda_160

Wanda_161

Wanda_162
(MazG-like protein)

Wanda_163

Wanda_164

Wanda_165
(metallophosphatase)

Wanda_166

Wanda_167

Wanda_168

Wanda_169

Wanda_170

Wanda_171

Wanda_172

Wanda_173
(metallophosphoesterase)

Wanda_174

Wanda_175

Wanda_176

Wanda_177

Wanda_178

Wanda_179

Wanda_180

Wanda_182

Wanda_183

Wanda_184
(HIT family domain protein)

Wanda_185

Wanda_186

Wanda_187

Wanda_188

Wanda_189

Wanda_190

Wanda_191

Wanda_192

Wanda_193

Wanda_194

Wanda_195

Wanda_196
(FtsK)

Wanda_197

Wanda_198

Wanda_199

Wanda_200
(Ku protein)

Wanda_201

Wanda_202

Wanda_203

Wanda_204

Wanda_205

Wanda_206

Wanda_207

Wanda_208

Wanda_209

Wanda_210

Wanda_211

Wanda_212

Wanda_213

Wanda_214

Wanda_215

Wanda_216

Wanda_217

Wanda_218

Wanda_219

Wanda_220

Wanda_221

Wanda_222

Wanda_223

Wanda_224

Wanda_225

Wanda_226

Wanda_227

Wanda_228

Wanda_229

Wanda_230

Wanda_231

Wanda_232

Wanda_233

Wanda_234

Wanda_235

Wanda_236

Wanda_237

Wanda_238

Wanda_239

Wanda_240

Wanda_241

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF006818
Refseq NumberNC_022067
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 25
Pitt Freezer Box Grid# 22
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload