| Detailed Information for Phage Juicebox | |
| Discovery Information | |
| Isolation Host | Corynebacterium xerosis ATCC 373 |
| Found By | Aneesh Pathak and Ote Staton |
| Year Found | 2017 |
| Location Found | Birmingham, AL USA |
| Finding Institution | University of Alabama at Birmingham |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.543682 N, 86.779633 W Map |
| Discovery Notes | Sewage influent. The isolation temperature was initially 30 C, but plaques took 72-120 hours to appear. When we moved to the 37 C temperature, the plaques appeared within 48 hours, so we continued to isolate/incubate at 37 C for the sake of time. |
| Naming Notes | The name comes from the hit movie Kicking and Screaming starring Will Ferrell and NFL Hall of Fame coach Mike Ditka. This movie was influential in our childhoods so we call our phage Juicebox to commemorate our separate but similar pasts. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 21, 2017 |
| Genome length (bp) | 41394 |
| Character of genome ends | Unknown |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAGGGCAT |
| GC Content | 67.9% |
| Sequencing Notes | at position 3208 bp approximately 25% of reads contain an A instead of consensus G |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to ViewArV2 Attoomi Barebow Cantare ChewyVIII Chymera CyranoPS DocB7 DrWorm DS6A E3 Elener EmiRose Eyre Finch Finkle Footloose FuzzBuster GAL1 Gilgamesh GMA1 GMA4 Gudmit Ibantik IdentityCrisis Jace JeanGrey Juicebox Kithara Kromp Kumao LilSpotty LuckyBarnes Magritte Min1 MooMoo mu16 OnionKnight P3MA PensacolaC28 phiAsp2 phiSAV Phroglets Pine5 Ponzi PSonyx pZL12 REQ2 REQ3 Reynauld RomansRevenge Root RRH1 Samy Shambre1 Shocker Shrew Sleepyhead Spartoi StAugustine Success Sunfish TinyDot TJE1 Tonitrus TPA2 TPA4 Trogglehumper TurkishDelight ValentiniPuff VanLee Whack Wollypog Yappy |
| Annotating Institution | University of Alabama at Birmingham |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are consistently small (0.5mm diameters) and cloudy. |
| Morphotype | Siphoviridae |
| Number of Genes | 59 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewJuicebox_1 Juicebox_2 Juicebox_3 Juicebox_4 Juicebox_5 Juicebox_6 Juicebox_7 Juicebox_8 Juicebox_9 Juicebox_10 Juicebox_11 Juicebox_12 Juicebox_13 Juicebox_14 Juicebox_15 Juicebox_16 Juicebox_17 Juicebox_18 Juicebox_19 Juicebox_20 Juicebox_21 Juicebox_22 Juicebox_23 Juicebox_24 Juicebox_25 Juicebox_26 Juicebox_27 Juicebox_28 Juicebox_29 Juicebox_30 Juicebox_31 Juicebox_32 Juicebox_33 Juicebox_34 Juicebox_35 Juicebox_36 Juicebox_37 Juicebox_38 Juicebox_39 Juicebox_40 Juicebox_41 Juicebox_42 Juicebox_43 Juicebox_44 Juicebox_45 Juicebox_46 Juicebox_47 Juicebox_48 Juicebox_49 Juicebox_50 Juicebox_51 Juicebox_52 Juicebox_53 Juicebox_54 Juicebox_55 Juicebox_56 Juicebox_57 Juicebox_58 Juicebox_59 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727550 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 59 |
| Pitt Freezer Box Grid# | E2 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |