| Detailed Information for Phage JuicyJay |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emmett Askira |
| Year Found | 2015 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.828991 N, 71.399643 W Map |
| Discovery Notes | Soil originated from a planter on the Southeast corner of Brook and Meeting streets that contained a mixture of soil and mulch, with some roots. The sample was drawn from the surface to about 3cm of depth. The temperature at the time of collection was 21C. JuicyJay was found using direct plating. |
| Naming Notes | The phage is predicted to be of cluster J. A musical artist has a similar name, but spelled differently, and declined the invitation to have phage named after him. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1007 |
| Genome length (bp) | 113075 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Webster University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are cloudy, and of widely variable size. In some, the cloudiness is evenly distributed in a ring, while in others there is a tail, resembling a short comet's tail of cloudiness in one direction, while the other edge is fairly defined. Plaques range from less than 0.5mm diameter to a maximum around 4mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 235 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ958748 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
2.1 x 10^10 |
| Date of SEA Lysate Titering |
Oct 23, 2015 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
