Mycobacterium phage Lucky2013
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Detailed Information for Phage Lucky2013
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByWest MIfflin High School Students
Year Found2012
Location FoundWest Mifflin, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.381647N, 79.873422 W Map
Discovery NotesA student from West Mifflin Area High School found the phage.
Naming NotesThe student decided to name the phage in honor of their graduating class (2013).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 14, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage599
Sanger Finishing Reactions9
Genome length (bp)108627
Character of genome ends3' Sticky Overhang
Overhang Length4 bases
Overhang SequenceATCC
GC Content61.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterJ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes237
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Lucky2013_1
(HNH endonuclease)

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(terminase)

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(portal protein)

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(capsid maturation protease)

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(major capsid protein)

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(glycosyltransferase)

Lucky2013_16
(serine/threonine kinase)

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(glycosyltransferase)

Lucky2013_18
(glycosyltransferase)

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(glycosyltransferase)

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(head-to-tail adaptor)

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(head-to-tail stopper)

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(tail terminator)

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(major tail protein)

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(tail assembly chaperone)

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(tail assembly chaperone)

Lucky2013_32
(tape measure protein)

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(minor tail protein)

Lucky2013_34
(minor tail protein)

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(minor tail protein)

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(minor tail protein)

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(minor tail protein)

Lucky2013_38
(minor tail protein)

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Lucky2013_46
(HNH endonuclease)

Lucky2013_47
(lysin A)

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(lysin B)

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Lucky2013_50

Lucky2013_51
(thioredoxin)

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Lucky2013_57
(Lsr2-like DNA bridging protein)

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Lucky2013_83
(excise)

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(tyrosine integrase)

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Lucky2013_90
(helix-turn-helix DNA binding protein)

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(esterase)

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Lucky2013_109
(RecB-like exonuclease)

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Lucky2013_113
(DNA methylase)

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Lucky2013_121
(polynucleotide kinase)

Lucky2013_122

Lucky2013_123
(endonuclease VII)

Lucky2013_124
(WhiB family transcription factor)

Lucky2013_125
(helix-turn-helix DNA binding protein)

Lucky2013_126

Lucky2013_127

Lucky2013_128

Lucky2013_129

Lucky2013_130
(DNA primase/polymerase)

Lucky2013_131

Lucky2013_132

Lucky2013_133

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Lucky2013_135

Lucky2013_136
(helix-turn-helix DNA binding protein)

Lucky2013_137

Lucky2013_138

Lucky2013_139

Lucky2013_140
(DNA primase/helicase)

Lucky2013_141

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Lucky2013_143

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Lucky2013_146

Lucky2013_147

Lucky2013_148
(RNA ligase)

Lucky2013_149
(ClpP-like protease)

Lucky2013_150
(DnaQ-like DNA polymerase III subunit)

Lucky2013_151

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Lucky2013_156

Lucky2013_157

Lucky2013_158
(PAPS reductase-like protein)

Lucky2013_159

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Lucky2013_161
(nucleotide pyrophosphohydrolase)

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Lucky2013_180 — tRNA
(tRNA- Gly (tcc))

Lucky2013_181

Lucky2013_182

Lucky2013_183 — tRNA
(tRNA-Tyr (gta))

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Lucky2013_196
(FtsK-like DNA translocase)

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Lucky2013_199

Lucky2013_200
(Ku-like dsDNA break-binding protein)

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Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF133445
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 3
Pitt Freezer Box Grid# F11
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload