| Detailed Information for Phage Luthien | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Kirana Bahls |
| Year Found | 2025 |
| Location Found | Montrose, WI United States of America |
| Finding Institution | Madison Area Technical College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.920527 N, 89.558948 W Map |
| Discovery Notes | The soil sample was collected from a compost pile with the help of my four-year-old sister Rania. The compost, taken from 2-8 centimeters below the ground, was filled with pumpkin shell and seed fragments. It was collected on 2025-10-06 at 8am, and the temperature was 19 Celsius. Isolation commenced the same day at 1pm. After two rounds of purification, two phage were discovered in the same sample, differentiated by different plaque sizes. This is the smaller of the two; the other is named Beren. |
| Naming Notes | Luthien (Sindarin for "daughter of flowers") was the daughter of the Maia Melian. Her secondary name, Tinuviel, means "nightingale." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1017 |
| Genome length (bp) | 61258 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAflac AlainaMarie Aloe25 Arataki ArtetaBall Artorias Avazak BetterYeti Blanky BluerMoon Bugg Burley Captrips Capybara ChickyNuggy Chikenjars Citrus Conley Crocheter CWormy Dancer Demure Dialga Duffington Eddiemania EndAve Fazel Figliar Football FortCran Gill Glintora Gravy Gusicorn Hortihabitatio Hydrus Jodelie19 JulesRay Kenosha Kerry Luthien Madvan Magel MajinBuu Mossy Mysticmoon Nadmeg Nithya NoPickles Odesza OhMyWard OlgasClover Perkunas PestoPenguin Petito Phepper Pherobrine Rickmore Roney Runhaar Schwartz33 Secretariat ShaggyRogers Tairn4Wing Talon44 Tanis TenaciousP Tonaltzin Trufflozitus Untouchable Vardy Zeph |
| Annotating Institution | Madison Area Technical College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The plaques are approximately 1mm in diameter. The edges are not very crisp. |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLuthien_1 Luthien_2 Luthien_3 Luthien_4 Luthien_5 Luthien_6 Luthien_7 Luthien_8 Luthien_9 Luthien_10 Luthien_11 Luthien_12 Luthien_13 Luthien_14 Luthien_15 Luthien_16 Luthien_17 Luthien_18 Luthien_19 Luthien_20 Luthien_21 Luthien_22 Luthien_23 Luthien_24 Luthien_25 Luthien_26 Luthien_27 Luthien_28 Luthien_29 Luthien_30 Luthien_31 Luthien_32 Luthien_33 Luthien_34 Luthien_35 Luthien_36 Luthien_37 Luthien_38 Luthien_39 Luthien_40 Luthien_41 Luthien_42 Luthien_43 Luthien_44 Luthien_45 Luthien_46 Luthien_47 Luthien_48 Luthien_49 Luthien_50 Luthien_51 Luthien_52 Luthien_53 Luthien_54 Luthien_55 Luthien_56 Luthien_57 Luthien_58 Luthien_59 Luthien_60 Luthien_61 Luthien_62 Luthien_63 Luthien_64 Luthien_65 Luthien_66 Luthien_67 Luthien_68 Luthien_69 Luthien_70 Luthien_71 Luthien_72 Luthien_73 Luthien_74 Luthien_75 Luthien_76 Luthien_77 Luthien_78 Luthien_79 Luthien_80 Luthien_81 Luthien_82 Luthien_83 Luthien_84 Luthien_85 Luthien_86 Luthien_87 Luthien_88 Luthien_89 Luthien_90 Luthien_91 Luthien_92 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 9.4 x 10^10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
