| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cole Blair |
| Year Found | 2011 |
| Location Found | Sandy Hook, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.076844 N, 83.183492 W Map |
| Discovery Notes | The sample came from a creek at approximately sixty degrees fahrenheit. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 30, 2012 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 400 |
| Sanger Finishing Reactions | 5 |
| Genome length (bp) | 55178 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCTGA |
| GC Content | 62.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques form a sort of "halo" ring around their darker centers. They grow close together and towards each other, but the rings of each never touch each other. They were about 5mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH001449 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WEKUMooMooBCB |
| SEA Lysate Titer |
4.4e12 |
| Date of SEA Lysate Titering |
Oct 11, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
