| Detailed Information for Phage NihilNomen |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Astha Mehta, Ryan Doss |
| Year Found | 2018 |
| Location Found | Las Vegas, NV USA |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.113974 N, 115.144462 W Map |
| Discovery Notes | Soil sample taken from plot 28 at UNLV community gardens. The soil sample was taken from 6 cm below surface. There was heavy moisture levels and dark soil color present that day. |
| Naming Notes | The original name of the phage was NoName because we could not come up with a name. However, NoName was taken and so we choose to change NoName to latin. Therefore, the phage was named NihilNomen. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 897 |
| Genome length (bp) | 110439 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques vary from 0.5mm up to 2mm in size. There also seems to be a partial clearing of 2-4 mm extra. Thus, making the phages lysogenic. |
| Morphotype | Siphoviridae |
| Number of Genes | 240 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK967402 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
78 |
| Pitt Freezer Box Grid# |
B6 |
| Available Files |
| GenBank File for Phamerator | Download |