| Detailed Information for Phage Phroglets | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Rakshaa Venkatraman |
| Year Found | 2024 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.021904 N, 118.401896 W Map |
| Discovery Notes | Depth from which sample was taken: 3cm Air temperature if sample collected outdoors (in degrees Celsius): 26.67ºC Direct Plating or Enrichment: Enrichment Enrichment protocol used (if any): Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. Additional information of interest or note: area was mostly dry soil but the specific area from where collected was wet (evident water spill, trash nearby, lots of litter like water bottles, gum, etc. in area) AL Quarter and Lab section (ie. F19-1A): F24-1C Instructor: Dr. Tejas Bouklas, Dr. Daniel Velez-Ramirez Teaching Assistant: Ally Aves Undergraduate Assistant(s): Jolie Vo, Eleanor Gorham Team #1 Name: Phrogs Team #1 Members: Rakshaa Venkatraman, Emily Ea, Alette Laughton, Bashar Allataifih |
| Naming Notes | Named in honor of team Phrogs for their love for frogs. The phage also doesn’t have a noticeable tail from TEM imaging, similar to how frogs absorb their tail during metamorphosis. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3644 |
| Genome length (bp) | 47231 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1324 bp |
| End Determination Notes | There is a region of ~twofold coverage compared to the rest of the genome, with clear read start buildups at either end, indicating a terminal repeat of length 1324 bp. |
| GC Content | 43.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to ViewArV2 Attoomi Ball Barebow Cantare ChewyVIII Chymera CyranoPS DillyDally DocB7 DrWorm DS6A E3 Elener EmiRose Eyre Finch Finkle Footloose FuzzBuster GAL1 Gilgamesh GMA1 GMA4 Gudmit Ibantik IdentityCrisis Jace JeanGrey Juicebox Kithara Kromp Kumao LilSpotty LuckyBarnes Lukepolites Magritte Min1 MooMoo mu16 OnionKnight P3MA PensacolaC28 phiAsp2 phiSAV Phroglets Pine5 Ponzi PSonyx pZL12 REQ2 REQ3 Reynauld RomansRevenge Root RRH1 Samy Shambre1 Shocker Shrew Sleepyhead Spartoi StAugustine Sunfish TinyDot TJE1 Tonitrus TPA2 TPA4 Trogglehumper TurkishDelight ValentiniPuff VanLee Whack Wollypog Yappy |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Description of plaque morphology: pinpoint plaques, fuzzy edges, turbid Average size (and size range) diameter in mm: 0.75-1 mm |
| Morphotype | Podoviridae |
| Number of Genes | 61 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPhroglets_1 Phroglets_2 Phroglets_3 Phroglets_4 Phroglets_5 Phroglets_6 Phroglets_7 Phroglets_8 Phroglets_9 Phroglets_10 Phroglets_11 Phroglets_12 Phroglets_13 Phroglets_14 Phroglets_15 Phroglets_16 Phroglets_17 Phroglets_18 Phroglets_19 Phroglets_20 Phroglets_21 Phroglets_22 Phroglets_23 Phroglets_24 Phroglets_25 Phroglets_26 Phroglets_27 Phroglets_28 Phroglets_29 Phroglets_30 Phroglets_31 Phroglets_32 Phroglets_33 Phroglets_34 Phroglets_35 Phroglets_36 Phroglets_37 Phroglets_38 Phroglets_39 Phroglets_40 Phroglets_41 Phroglets_42 Phroglets_43 Phroglets_44 Phroglets_45 Phroglets_46 Phroglets_47 Phroglets_48 Phroglets_49 Phroglets_50 Phroglets_51 Phroglets_52 Phroglets_53 Phroglets_54 Phroglets_55 Phroglets_56 Phroglets_57 Phroglets_58 Phroglets_59 Phroglets_60 Phroglets_61 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
