| Detailed Information for Phage Reynauld |
| Discovery Information |
| Isolation Host | Rhodococcus equi NRRL B-16538 |
| Found By | Calvin Ritzema |
| Year Found | 2022 |
| Location Found | Grand Rapids, MI United States of America |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.893611 N, 85.738611 W Map |
| Discovery Notes | Reynauld was adopted from Prof Wertz. He found it in the back corner of his yard where he said he had buried a dead rabbit about 3 years ago. He described the soil as being dry and “loamy” – a mixture of clay, sand, and humus. Collected early morning, about 65 degrees and cloudy. |
| Naming Notes | I was playing a video game called Darkest Dungeon and there was a character named Reynauld. So I named it Reynauld. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 14, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3099 |
| Genome length (bp) | 80980 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 362 bp |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Calvin University |
| Annotation Status | In GenBank |
| Plaque Notes | Small pencil tip size. Clear and stretched out at the edges. Size approximately 2mm each. |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR159659 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
162 |
| Pitt Freezer Box Grid# |
F6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
