Arthrobacter phage RomansRevenge
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| Detailed Information for Phage RomansRevenge | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Kylie Tseng |
| Year Found | 2023 |
| Location Found | Los Angeles, CA US |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.06202 N, 118.29652 W Map |
| Discovery Notes | Isolation by Dominic Garza and Michelle Zorawik. An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in equal concentrations. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method. Characterization by Team PhageFright. Milena Deal, Nicole Critzer, Thomas Kretschmer, Griffin Gowdy, Audia Hughes AL Quarter and Lab section: W23-1B Instructor: Amanda Freise Teaching Assistant: Dom Garza Undergraduate Assistant(s): Melvin and Sophia |
| Naming Notes | The name is a direct reference to a song on Nicki Minaj’s debut studio album titled Pink Friday. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 19, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 293 |
| Genome length (bp) | 62027 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1042 bp |
| End Determination Notes | As this was the first phage of its (close) kind, we looked closely at the ends. There was a clear region of about 1 kb that had ~2x the coverage level of the rest of the genome. This implies a direct terminal repeat. The right end of this terminal repeat was well-defined in the sequencing reads, with many read starts appearing at a particular base. The left end was less well-defined, however, and so we chose a base near the left end of the double-coverage region that had slightly more read starts than the surrounding area. |
| GC Content | 60.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Awaiting_QC |
| Plaque Notes | Very turbid with pinpoint clear centers. 1 mm |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 184 |
| Pitt Freezer Box Grid# | E9 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
