| Detailed Information for Phage Samy | |
| Discovery Information | |
| Isolation Host | Streptomyces ambofaciens ATCC 23877 |
| Found By | Stéphanie Bury-Moné |
| Year Found | 2023 |
| Location Found | Péronne, France |
| Finding Institution | University Paris-Saclay |
| Program | Unavailable |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 49.93333 N, 2.93333 E Map |
| Discovery Notes | Samy is a temperate phage present in the genome of Streptomyces ambofaciens ATCC 23877. Samy particles were isolated from cultures of this strain grown for 4 days at 30°C in BM medium (1 g/l yeast extract, 1 g/l meat/beef extract, 2 g/l bactotryptone, pH 7.3). Samy is produced produced simultaneously with the activation of other mobile genetic elements and phage-related genes such as those encoding phage tail-like nanostructures. Publication: Jaffal H, Kortebi M, Misson P, Tavares P, Ouldali M, Leh H, Lautru S, Lioy VS, Lecointe F, Bury-Moné SG. Prophage induction can facilitate the in vitro dispersal of multicellular Streptomyces structures. PLoS Biol. 2024 Jul 25;22(7):e3002725. doi: 10.1371/journal.pbio.3002725. PMID: 39052683. |
| Naming Notes | The name was chosen because of the name of the host strain Streptomyces ambofaciens (SAM) ATCC 23877, which already contains other mobile genetic elements, plasmids called pSAM1 and pSAM2. The phage produced was therefore named Samy. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2024 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2558 |
| Genome length (bp) | 61068 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGTTAAGGTGC |
| GC Content | 66.5% |
| Sequencing Notes | There is one base in the PhagesDB genome of this sequence that differs from the sequence currently in GenBank. At position 55286, we have a G where the GenBank file has a T. This is because the sequencing reads showed a polymorphism at that position, and we called a G (~59% of reads) whereas the previous assembly called a T (~41% of reads). Neither is "right" or "wrong", as the population that was sequenced was truly mixed at this position. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to ViewArV2 Attoomi Barebow Cantare ChewyVIII Chymera CyranoPS DocB7 DrWorm DS6A E3 Elener EmiRose Eyre Finch Finkle Footloose FuzzBuster GAL1 Gilgamesh GMA1 GMA4 Gudmit Ibantik IdentityCrisis Jace JeanGrey Juicebox Kithara Kromp Kumao LilSpotty LuckyBarnes Magritte Min1 MooMoo mu16 OnionKnight P3MA PensacolaC28 phiAsp2 phiSAV Pine5 Ponzi PSonyx pZL12 REQ2 REQ3 Reynauld RomansRevenge Root RRH1 Samy Shambre1 Shocker Shrew Sleepyhead Spartoi StAugustine Success Sunfish TinyDot TJE1 Tonitrus TPA2 TPA4 Trogglehumper TurkishDelight ValentiniPuff VanLee Whack Wollypog Yappy |
| Annotating Institution | University Paris-Saclay |
| Annotation Status | In GenBank |
| Plaque Notes | Very small plaques (< 1 mm). Lab host: Streptomyces lividans TK24 |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 4 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSamy_1 Samy_2 Samy_3 Samy_4 Samy_5 Samy_6 Samy_7 Samy_8 Samy_9 Samy_10 Samy_11 Samy_12 Samy_13 Samy_14 Samy_15 Samy_16 Samy_17 Samy_18 Samy_19 Samy_20 Samy_21 Samy_22 Samy_23 Samy_24 Samy_25 Samy_26 Samy_27 Samy_28 Samy_29 Samy_30 Samy_31 Samy_32 Samy_33 Samy_34 Samy_35 Samy_36 Samy_37 Samy_38 Samy_39 Samy_40 Samy_41 Samy_42 Samy_43 Samy_44 Samy_45 Samy_46 Samy_47 Samy_48 Samy_49 Samy_50 Samy_51 Samy_52 Samy_53 Samy_54 Samy_55 Samy_56 Samy_57 Samy_58 Samy_59 Samy_60 Samy_61 Samy_62 Samy_63 Samy_64 Samy_65 Samy_66 Samy_67 Samy_68 Samy_69 Samy_70 Samy_71 Samy_72 Samy_73 Samy_74 Samy_75 Samy_76 Samy_77 Samy_78 Samy_79 Samy_80 Samy_81 Samy_82 Samy_83 Samy_84 Samy_85 Samy_86 Samy_87 Samy_88 Samy_89 Samy_90 Samy_91 Samy_92 Samy_93 Samy_94 Samy_95 Samy_96 — tRNA Samy_97 — tRNA Samy_98 — tRNA Samy_99 — tRNA Samy_100 Samy_101 Samy_102 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR263580 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
