| Detailed Information for Phage ThreeRngTarjay |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alex Jackman and Nour El Yaman |
| Year Found | 2016 |
| Location Found | Detroit, MI USA |
| Finding Institution | University of Detroit Mercy |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.414 N, 83.137982 W Map |
| Discovery Notes | This phage was discovered in a flowerbed filled with mulch in front of Shiple Hall on the University of Detroit Mercy campus. |
| Naming Notes | The original name of this phage was Three Ring Target. It was named this because the phage reliably forms plaques that have three distinct rings of bacteria that have not been lysed, around a center of lytic phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 27, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 350 |
| Genome length (bp) | 113254 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 61.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Detroit Mercy |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 238 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524527 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.5*10^(9) |
| Date of SEA Lysate Titering |
Nov 15, 2016 |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
B6 |
| Available Files |
| GenBank File for Phamerator | Download |