Mycobacterium phage Xiaokay
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| Detailed Information for Phage Xiaokay | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kaylee Mak-lin |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 45.317884 N, 75.603949 W Map |
| Discovery Notes | Collected 2 cm under a tree in front of a school in slightly damp, and dark soil with some wood chips. Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry. The sequence assembled into one contig of 40,900 bp with 97% query cover, 99.94% identity to Constella (J). The sequence was assembled into a second shorter contig of 30,774 bp with 90% query cover, 99.41% identity to Constella (J). This sequence is incomplete based on the size of other J phages. |
| Naming Notes | The name takes inspiration from my username in games, Xiao meaning "small" in Chinese, much like me and my phage, while kay is the first part of my name. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 27, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 709 |
| Genome length (bp) | 110277 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAriel Bagrid BAKA Beem Bobby Bombitas Constella Courthouse Dallas DmpstrDiver Dove Duke13 Ejimix EricMillard Gonephishing Halley Hannaconda Hidrated HokkenD Hughesyang JuicyJay Kalah2 KashFlow Klein LittleE Lucky2013 Marleymoo MiaZeal Minerva Nekros NihilNomen Odette Omega Optimus Phoebus Porcelain Pound Rearden Redno2 Schatzie Shaboozey Squint Superphikiman Thibault ThreeRngTarjay Wanda Xiaokay Yeet Zelink |
| Annotating Institution | University of Ottawa |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Plaque sizes are small, ranging from 0.5mm-1mm, slightly turbid borders with a clear center. Plaques have defined edges. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |