| Detailed Information for Phage AvGardian |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Garrett Steele & Avery Cline |
| Year Found | 2019 |
| Location Found | Statesville, NC United States of America |
| Finding Institution | Mitchell Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 35.7833 N, 80.8972 W Map |
| Discovery Notes | A huge thank you to Melissa Simmons for letting us take a sample from her garden, which is fertilized with chicken waste. |
| Naming Notes | The name is a mixture of both of the lab partners that worked on the phage, and combined with the word 'guardian'. Av=Avery, Gar=Garrett |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 25, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1080 |
| Genome length (bp) | 41522 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Mitchell Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Our individual plaques seemed to possess a 'halo', or a lighter outside ring that surrounded each plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT310853 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.53x10^10 |
| Pitt Freezer Box# |
97 |
| Pitt Freezer Box Grid# |
B2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
