Microbacterium phage Burritobowl
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Detailed Information for Phage Burritobowl
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found BySara Hughes and Mahnoor Siddiqui
Year Found2019
Location FoundLanghorne, PA United States of America
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.13534 N, 74.8908 E Map
Discovery NotesDiscovered Next to a barbecue grill in a suburban area. The soil was moist, and it was dug up about 10 cm below the ground. The temperature outside was about 25 degrees Celsius when the soil was collected.
Naming NotesWe chose this name for our Phage due to the fact that “Burrito” was already taken as name for another Phage in the database. We found that this name was very cute as catchy, and it was the first thing to come to our minds.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 20, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage40
Genome length (bp)41417
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content66.5%
Sequencing NotesThis phage was sequenced as part of a DOGEMS sample.
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesOur plaques are about 0.635 cm in both heigh and width. They are both Turgid and Bulls-eye.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 3.4X10^6 PFU/mL
Available Files
Plaque PictureDownload
EM PictureDownload