Microbacterium phage Johnathan
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Detailed Information for Phage Johnathan
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByMackenzie Horutz
Year Found2021
Location FoundWI Kenosha
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature24°C
GPS Coordinates42.6201 N, 87.8218 W Map
Discovery NotesThe soil sample was originally found by Logan Seery, and I adopted a phage from one of his initial plates because my sample did not yield any results. I was partially assisted by Jeanelle Young for the lab procedures.
Naming NotesIn all honesty, I did not know what to name my phage. I decided to go with something random, and picked what Paul Rudd said the name of Thor's hammer in an interview once.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 26, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage697
Genome length (bp)40971
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionCarthage College
Annotation StatusIn GenBank
Plaque NotesThe plaques were of a consistent small size and were rounded in shape.
Has been Phamerated?Yes
Gene List

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Johnathan_1

Johnathan_2
(terminase, small subunit)

Johnathan_3
(terminase, large subunit)

Johnathan_4
(portal protein)

Johnathan_5
(capsid maturation protease)

Johnathan_6
(major capsid protein)

Johnathan_7

Johnathan_8
(head-to-tail adaptor)

Johnathan_9
(head-to-tail stopper)

Johnathan_10

Johnathan_11
(tail terminator)

Johnathan_12
(major tail protein)

Johnathan_13
(tail assembly chaperone)

Johnathan_14
(tail assembly chaperone)

Johnathan_15

Johnathan_16
(tape measure protein)

Johnathan_17
(minor tail protein)

Johnathan_18
(minor tail protein)

Johnathan_19

Johnathan_20
(minor tail protein)

Johnathan_21
(minor tail protein)

Johnathan_22
(endolysin)

Johnathan_23
(holin)

Johnathan_24

Johnathan_25

Johnathan_26

Johnathan_27

Johnathan_28
(Cas4 exonuclease)

Johnathan_29

Johnathan_30

Johnathan_31
(MazG-like nucleotide pyrophosphohydrolase)

Johnathan_32
(thymidylate kinase)

Johnathan_33
(recombination directionality factor)

Johnathan_34

Johnathan_35
(thioredoxin)

Johnathan_36
(Holliday junction resolvase)

Johnathan_37

Johnathan_38

Johnathan_39

Johnathan_40
(DNA primase/helicase)

Johnathan_41
(DNA polymerase I)

Johnathan_42

Johnathan_43

Johnathan_44
(DNA binding protein)

Johnathan_45

Johnathan_46

Johnathan_47

Johnathan_48
(ParB-like nuclease domain)

Johnathan_49

Johnathan_50

Johnathan_51
(HNH endonuclease)

Johnathan_52

Johnathan_53

Johnathan_54

Johnathan_55

Johnathan_56
(dihydrofolate reductase)

Johnathan_57

Johnathan_58

Johnathan_59
(thymidylate synthase)

Johnathan_60

Johnathan_61

Johnathan_62

Johnathan_63

Johnathan_64

Johnathan_65

Johnathan_66

Johnathan_70
(HNH endonuclease)

Johnathan_71

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP405128
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 137
Pitt Freezer Box Grid# C12
Available Files
GenBank File for PhameratorDownload