Microbacterium phage Stromboli
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Detailed Information for Phage Stromboli
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByTimothy Abbott
Year Found2018
Location FoundNew Haven, CT USA
Finding InstitutionSouthern Connecticut State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates41.3345 N, 72.9429 W Map
Discovery NotesFound in moist topsoil near brush, approximately 18.3 degrees celsius and humid.
Naming NotesThe name for this phage came from a group of relatively unknown mobsters who traveled the country from coast to coast only to disappear in Oregon.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2212
Genome length (bp)41594
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content68.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionSouthern Connecticut State University
Annotation StatusIn GenBank
Plaque NotesPlaques form with a ~1mm diameter clear center at 24 hours and form an additional cloudy outer ring for a diameter of ~3mm at 48 hours.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Stromboli_1

Stromboli_2
(terminase, small subunit)

Stromboli_3
(terminase, large subunit)

Stromboli_4
(portal protein)

Stromboli_5
(capsid maturation protease)

Stromboli_6
(major capsid protein)

Stromboli_7

Stromboli_8
(head-to-tail adaptor)

Stromboli_9
(head-to-tail stopper)

Stromboli_10

Stromboli_11
(tail terminator)

Stromboli_12
(major tail protein)

Stromboli_13
(tail assembly chaperone)

Stromboli_14
(tail assembly chaperone)

Stromboli_15

Stromboli_16
(tape measure protein)

Stromboli_17
(minor tail protein)

Stromboli_18
(minor tail protein)

Stromboli_19
(minor tail protein)

Stromboli_20
(minor tail protein)

Stromboli_21
(lysin A)

Stromboli_22
(membrane protein)

Stromboli_23

Stromboli_24
(membrane protein)

Stromboli_25

Stromboli_26

Stromboli_27

Stromboli_28
(exonuclease)

Stromboli_29

Stromboli_30

Stromboli_31

Stromboli_32
(toxin in toxin/antitoxin system, HicA-like)

Stromboli_33
(deoxyuridine triphosphatase)

Stromboli_34
(thymidylate kinase)

Stromboli_35

Stromboli_36

Stromboli_37
(NrdH-like glutaredoxin)

Stromboli_38
(Holliday junction resolvase)

Stromboli_39

Stromboli_40

Stromboli_41

Stromboli_42
(DNA primase)

Stromboli_43
(DNA polymerase I)

Stromboli_44

Stromboli_45

Stromboli_46
(helix-turn-helix DNA binding domain)

Stromboli_47

Stromboli_48

Stromboli_49
(ParB-like nuclease domain)

Stromboli_50

Stromboli_51

Stromboli_52
(HNH endonuclease)

Stromboli_53

Stromboli_54

Stromboli_55

Stromboli_56

Stromboli_57
(dihydrofolate reductase)

Stromboli_58
(thymidylate synthase)

Stromboli_59
(membrane protein)

Stromboli_60

Stromboli_61

Stromboli_62

Stromboli_63

Stromboli_64

Stromboli_65

Stromboli_68
(HNH endonuclease)

Stromboli_69
(membrane protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT024860
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4x10^10
Pitt Freezer Box# 95
Pitt Freezer Box Grid# H10
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload