Microbacterium phage DirtyBubble
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Detailed Information for Phage DirtyBubble
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByEddie Muschio and Andrew Moonan
Year Found2018
Location FoundWalden, NY USA
Finding InstitutionMount Saint Mary College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.571695 N, 74.204024 W Map
Discovery NotesFound it in the backyard of my house
Naming NotesName came from an episode of Spongebob, since the plates had the same color and smell of the Dirty Bubble
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1312
Genome length (bp)41692
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content68.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionMount Saint Mary College
Annotation StatusIn GenBank
Plaque NotesThe plaques are very small when comparing to others in the class. They are also very irregular in shape, and not a nice defined circle.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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DirtyBubble_1

DirtyBubble_2
(terminase small subunit)

DirtyBubble_3
(terminase large subunit)

DirtyBubble_4
(portal protein)

DirtyBubble_5
(capsid maturation protease)

DirtyBubble_6
(major capsid protein)

DirtyBubble_7

DirtyBubble_8
(head-to-tail adaptor)

DirtyBubble_9
(head-to-tail stopper)

DirtyBubble_10

DirtyBubble_11
(tail terminator)

DirtyBubble_12
(major tail protein)

DirtyBubble_13
(tail assembly chaperone)

DirtyBubble_14
(tail assembly chaperone)

DirtyBubble_15

DirtyBubble_16
(tape measure protein)

DirtyBubble_17
(minor tail protein)

DirtyBubble_18
(minor tail protein)

DirtyBubble_19
(minor tail protein)

DirtyBubble_20
(minor tail protein)

DirtyBubble_21
(lysin A)

DirtyBubble_22
(membrane protein)

DirtyBubble_23
(membrane protein)

DirtyBubble_24
(membrane protein)

DirtyBubble_25

DirtyBubble_26

DirtyBubble_27

DirtyBubble_28
(exonuclease)

DirtyBubble_29

DirtyBubble_30

DirtyBubble_31
(HicA-like toxin)

DirtyBubble_32
(deoxyuridine triphosphatase)

DirtyBubble_33
(thymidylate kinase)

DirtyBubble_34

DirtyBubble_35

DirtyBubble_36
(thioredoxin)

DirtyBubble_37
(holliday junction resolvase)

DirtyBubble_38

DirtyBubble_39

DirtyBubble_40

DirtyBubble_41
(DNA primase)

DirtyBubble_42
(DNA polymerase I)

DirtyBubble_43

DirtyBubble_44

DirtyBubble_45
(helix-turn-helix DNA binding domain protein)

DirtyBubble_46

DirtyBubble_47

DirtyBubble_48

DirtyBubble_49

DirtyBubble_50

DirtyBubble_51
(HNH endonuclease)

DirtyBubble_52

DirtyBubble_53

DirtyBubble_54

DirtyBubble_55

DirtyBubble_56
(thymidylate synthase)

DirtyBubble_57

DirtyBubble_58
(thymidylate synthase)

DirtyBubble_59
(membrane protein)

DirtyBubble_60

DirtyBubble_61

DirtyBubble_62

DirtyBubble_63

DirtyBubble_64

DirtyBubble_65

DirtyBubble_69
(HNH endonuclease)

DirtyBubble_70
(membrane protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN062714
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.29x10^11
Pitt Freezer Box# 80
Pitt Freezer Box Grid# E1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload