Microbacterium phage Doobus
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Detailed Information for Phage Doobus
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByDavid Ennest
Year Found2018
Location FoundRiver Falls, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates44.851966 N, 92.232589 W Map
Discovery NotesFound on 9-19-2018.
The soil was collected next to the Kinnickinnic river near the bridge on the trail that connects the falcon trail to the rest of campus.
Weather conditions when collected were clear with low humidity, and the temperature was 72°F.
The soil looked loose, dark, and very moist. It was collected at a depth of 25mm
Coordinates of collection site are : 44.851966 N, 92.232589 W
Naming NotesThe name is a conjunction of the words darn and goobus. This is because the phage is as weak as a darn goobus.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 18, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage41
Genome length (bp)41195
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content66.3%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionQueens University of Charlotte
Annotation StatusIn GenBank
Plaque NotesPlaques are minuscule in size and have very slight turbid halos.
Approximate size is that of a written period.
Uniform in size and shape
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ820083
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 87
Pitt Freezer Box Grid# G2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload