Microbacterium phage Gack
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Detailed Information for Phage Gack
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByGabrielle Stack
Year Found2023
Location FoundWillington, CT United States
Finding InstitutionUniversity of Connecticut
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature22°C
GPS Coordinates41.83765 N, 72.262379 W Map
Discovery NotesFound by dumpster near apartment complex - compact soil that is consistently dry and in the shade
Naming NotesThe name of this plaque originally came from a combination of my first and last name, however, the morphology of this plaque has an irregular edge, similar to a paint splatter, or goop, something that would make you exclaim GACK!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1387
Genome length (bp)40547
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceACTCCCGGCA
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesWe conducted a lysogeny test and it was found that this phage is lysogenic
Other Cluster Members
Annotating InstitutionUniversity of Connecticut
Annotation StatusAwaiting_QC
Plaque NotesPlaque morphology is different at different dilutions, with larger cloudy clearings appearing around the irregular center clearings at higher dilutions.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 183
Pitt Freezer Box Grid# A6
Available Files
Plaque PictureDownload
EM PictureDownload