Microbacterium phage Vitas
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Detailed Information for Phage Vitas
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByKatarzyna Waniolka
Year Found2018
Location FoundSaint Louis, MO USA
Finding InstitutionSt. Louis Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.568051 N, 90.420776 W Map
Discovery NotesPhage Vitas came from the soil sample collected on a rainy day (August 2018). The soil was collected from 2-3 inches deep under the roots of a bush and was mostly dry, brown to almost black, loose with some organic matter. The soil area had some access to the light, but partially shaded.
Naming NotesPhage Vitas was named from Latin word "vitas" that means "lives", because I was hoping that my phage will save the lives of others, and also because my phage (even though producing some halo) appears to be very active ("alive") with the host.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 30, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage692
Genome length (bp)39994
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceACTCCCGGCA
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionSt. Louis Community College
Annotation StatusIn GenBank
Plaque NotesVitas phage produces halo areas with double rings that are different in the turbidity. The phages tend to be very active with its host and in less concentrated numbers, some turbidity was seen. No cell formations were noticed in the plaques formed and the plates were consistent in the morphology. The plaques produced varied between 1mm to 3 mm and they were clear in the center, then turbid around, and if they grew larger they had one more ring around them.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN183281
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 76
Pitt Freezer Box Grid# D5
Available Files
GenBank File for PhameratorDownload