| Detailed Information for Phage Cashington |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Molly Fuchs Townzen, Jillian Hines |
| Year Found | 2023 |
| Location Found | Spokane, WA United States of America |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.66637 N, 117.40154 W Map |
| Discovery Notes | Found behind Gonzaga's library under a tree in winter. Soil was firm due to the the cold January temperatures. The weather upon discovery was 38-32 degrees Fahrenheit. The soil was packed tight and was silt-like. We also dug out our sample from under a bed of grass so we anticipated some organic material to be in our soil. |
| Naming Notes | Cashington is a combination of California and Washington - the two states we are from. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 30, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1764 |
| Genome length (bp) | 41600 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | Being Annotated (Expected completion by 7/1/2024) |
| Plaque Notes | Cashington’s plaque morphology includes small plaques roughly ~ 2 mm in diameter, and the plaques are slightly cloudy with fuzzy edges. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |