| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Ethan Dotzler, Jonathan Nyandu Kanyinda |
| Year Found | 2020 |
| Location Found | Moorhead, MN Clay |
| Finding Institution | Minnesota State University Moorhead |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -2°C |
| GPS Coordinates | 46.8673 N, 96.762 W Map |
| Discovery Notes | This sample was collected on January 27th, 2020. The conditions of the day were cold, with a temperature of 28*F. This sample was collected between two Pine trees out on the Minnesota State University Moorhead Campus Mall, with the sample being comprised of dirt that was collected from approximately 1 inch below the visible surface. |
| Naming Notes | We gratefully dedicate the name of our phage to the wonderful discoverer, Ethan Dotzler. This is the first of two samples found in relatively the same area, and it is now the youngest member of the Dotzler Family. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1387 |
| Genome length (bp) | 63076 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 217 bp |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Xavier University of Louisiana |
| Annotation Status | In GenBank |
| Plaque Notes | It was one of a few visible plaques on a series of plates, and was further isolated after collecting samples from those plaques. The Plaques were clear, and were relatively round in shape, somewhat in-between the shapes of a circle and an oval, but not quite either of the two. After further investigation, and thanks to the TEM photos received, we were able to identify this phage as a Siphoviridae. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR613478 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
6.67 X 10^10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
