Microbacterium phage Rudy
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Detailed Information for Phage Rudy
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByJack Mudge, Hannah Sparber
Year Found2018
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates47.666473 N, 117.403098 W Map
Discovery NotesSample was collected outside of the greenhouse in Hughes Hall, in dirt by a red berry bush. The soil was dark and slightly wet when collected.
Naming NotesWe chose the name Rudy after the main character in the movie "Rudy"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 7, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1209
Genome length (bp)61769
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length203 bp
GC Content67.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEG
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesThe plaques are consistently circular, mostly clear, with some slightly cloudy plaques, and 1-2mm in size.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Rudy_1

Rudy_2

Rudy_3

Rudy_4

Rudy_5

Rudy_6
(histidine triad nucleotide binding protein)

Rudy_7

Rudy_8
(DprA-like DNA processing chain A)

Rudy_9

Rudy_10

Rudy_11
(helix-turn-helix DNA binding domain protein)

Rudy_12
(helix-turn-helix DNA binding domain protein)

Rudy_13
(ribbon-helix-helix DNA binding domain protein)

Rudy_14
(helix-turn-helix DNA binding domain protein)

Rudy_15

Rudy_16

Rudy_17
(ribbon-helix-helix DNA binding domain protein)

Rudy_18
(terminase)

Rudy_19

Rudy_20
(portal protein)

Rudy_21
(capsid maturation protease and MuF-like fusion protein)

Rudy_22

Rudy_23
(major capsid protein)

Rudy_24

Rudy_25

Rudy_26
(major tail protein)

Rudy_27

Rudy_28
(phosphoesterase)

Rudy_29
(head-to-tail adaptor)

Rudy_30

Rudy_31

Rudy_32
(tail assembly chaperone)

Rudy_33
(tail assembly chaperone)

Rudy_34
(tape measure protein)

Rudy_35
(minor tail protein)

Rudy_36
(minor tail protein)

Rudy_37
(minor tail protein)

Rudy_38

Rudy_39

Rudy_40

Rudy_41

Rudy_42

Rudy_43
(endolysin)

Rudy_44

Rudy_45

Rudy_46
(DnaJ-like chaperonin)

Rudy_47

Rudy_48
(Cas4 family exonuclease)

Rudy_49
(RecA-like DNA recombinase)

Rudy_50
(HNH endonuclease)

Rudy_51
(RuvC-like resolvase)

Rudy_52

Rudy_53

Rudy_54
(MazG-like nucleotide pyrophosphohydrolase)

Rudy_55
(ssDNA binding protein)

Rudy_56

Rudy_57

Rudy_58

Rudy_59

Rudy_60

Rudy_61

Rudy_62

Rudy_63

Rudy_64

Rudy_65

Rudy_66

Rudy_67
(DNA primase/helicase)

Rudy_68

Rudy_69
(ribbon-helix-helix DNA binding domain protein)

Rudy_70

Rudy_71

Rudy_72

Rudy_73

Rudy_74

Rudy_75

Rudy_76

Rudy_77

Rudy_78

Rudy_79

Rudy_80

Rudy_81

Rudy_82

Rudy_83

Rudy_84

Rudy_85

Rudy_86

Rudy_87

Rudy_88

Rudy_89

Rudy_90

Rudy_91

Rudy_92

Rudy_93

Rudy_94

Rudy_95

Rudy_96

Rudy_97

Rudy_98

Rudy_99

Rudy_100

Rudy_101

Rudy_102

Rudy_103

Rudy_104

Rudy_105

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW365946
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 95
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload