Microbacterium phage SadLad
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Detailed Information for Phage SadLad
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Found ByMargaret Marx
Year Found2019
Location FoundNew Richmond, WI United States
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates45.2 N, 92.5 W Map
Discovery NotesFound in a home vegetable garden.
Naming NotesDue to many repeated procedures, I often found myself to be a sad lad. So I decided to share that burden with my sweet little phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 8, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage71
Genome length (bp)62801
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length211 bp
GC Content69.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEG
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionAustin Peay State University
Annotation StatusIn GenBank
Plaque Notessmall, clear, no halo, variable
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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SadLad_1

SadLad_2

SadLad_3

SadLad_4
(ParB-like nuclease domain)

SadLad_5

SadLad_6

SadLad_7
(histidine triad nucleotide binding protein)

SadLad_8

SadLad_9

SadLad_10
(DprA-like DNA processing chain A)

SadLad_11

SadLad_12

SadLad_13
(helix-turn-helix DNA binding domain)

SadLad_14
(DNA binding protein)

SadLad_15

SadLad_16

SadLad_17
(ribbon-helix-helix DNA binding domain)

SadLad_18

SadLad_19
(helix-turn-helix DNA binding domain)

SadLad_20

SadLad_21
(ribbon-helix-helix DNA binding domain)

SadLad_22
(terminase)

SadLad_23

SadLad_24

SadLad_25
(portal protein)

SadLad_26
(capsid maturation protease)

SadLad_27

SadLad_28
(HNH endonuclease)

SadLad_29
(major capsid protein)

SadLad_30

SadLad_31

SadLad_32
(major tail protein)

SadLad_33

SadLad_34
(esterase)

SadLad_35
(head-to-tail adaptor)

SadLad_36

SadLad_37

SadLad_38
(tail assembly chaperone)

SadLad_39
(tail assembly chaperone)

SadLad_40
(tape measure protein)

SadLad_41
(minor tail protein)

SadLad_42
(minor tail protein)

SadLad_43
(minor tail protein)

SadLad_44
(minor tail protein)

SadLad_45

SadLad_46

SadLad_47

SadLad_48
(endolysin)

SadLad_49

SadLad_50

SadLad_51

SadLad_52

SadLad_53
(chaperonin, DnaJ-like)

SadLad_54

SadLad_55
(MazG-like nucleotide pyrophosphohydrolase)

SadLad_56
(Cas4 family exonuclease)

SadLad_57
(DNA recombinase)

SadLad_58
(HNH endonuclease)

SadLad_59

SadLad_60
(RuvC-like resolvase)

SadLad_61

SadLad_62
(ssDNA binding protein)

SadLad_63

SadLad_64

SadLad_65

SadLad_66

SadLad_67

SadLad_68

SadLad_69
(helix-turn-helix DNA binding domain)

SadLad_70
(helix-turn-helix DNA binding domain)

SadLad_71
(DNA primase/helicase)

SadLad_72

SadLad_73

SadLad_74

SadLad_75

SadLad_76

SadLad_77

SadLad_78

SadLad_79
(MazG-like nucleotide pyrophosphohydrolase)

SadLad_80

SadLad_81

SadLad_82

SadLad_83

SadLad_84

SadLad_85

SadLad_86

SadLad_87

SadLad_88

SadLad_89

SadLad_90

SadLad_91

SadLad_92

SadLad_93

SadLad_94

SadLad_95

SadLad_96

SadLad_97

SadLad_98

SadLad_99

SadLad_100

SadLad_101

SadLad_102

SadLad_103

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW924641
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 102
Pitt Freezer Box Grid# H4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload