Microbacterium phage Wayne3
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Detailed Information for Phage Wayne3
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByDiana Hoppe, Karson Gjesdal
Year Found2021
Location FoundSpokane, WA United States
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature23°C
GPS Coordinates47.667432 N, 117.40321 W Map
Discovery NotesThis soil was collected from a mossy patch located near a curb and collected shortly after light rain. The soil was moist and soft.
Naming NotesThis is named after a construction worker on the Gonzaga campus who asked us about our research. We promised him we would name the phage after him.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage772
Genome length (bp)62371
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length199 bp
GC Content67.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEG
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesSize: .5-2mm
edges: fuzzy edges but short in width
Center Completely clear not turbid
Rings fuzzy border but no rings.
Shape: circular
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Wayne3_1

Wayne3_2

Wayne3_3

Wayne3_4

Wayne3_5

Wayne3_6

Wayne3_7
(histidine triad nucleotide binding protein)

Wayne3_8

Wayne3_9

Wayne3_10

Wayne3_11
(DprA-like DNA processing chain A)

Wayne3_12

Wayne3_13

Wayne3_14
(helix-turn-helix DNA binding domain protein)

Wayne3_15
(helix-turn-helix DNA binding domain protein)

Wayne3_16
(ribbon-helix-helix DNA binding domain protein)

Wayne3_17
(helix-turn-helix DNA binding domain protein)

Wayne3_18

Wayne3_19

Wayne3_20
(ribbon-helix-helix DNA binding domain protein)

Wayne3_21
(terminase)

Wayne3_22

Wayne3_23
(portal protein)

Wayne3_24
(capsid maturation protease)

Wayne3_25

Wayne3_26
(major capsid hexamer protein)

Wayne3_27

Wayne3_28

Wayne3_29
(major tail protein)

Wayne3_30

Wayne3_31
(phosphoesterase)

Wayne3_32
(head-to-tail adaptor)

Wayne3_33

Wayne3_34

Wayne3_35
(tail assembly chaperone)

Wayne3_36

Wayne3_37
(tape measure protein)

Wayne3_38
(minor tail protein)

Wayne3_39
(minor tail protein)

Wayne3_40
(minor tail protein)

Wayne3_41

Wayne3_42

Wayne3_43

Wayne3_44

Wayne3_45

Wayne3_46

Wayne3_47
(endolysin)

Wayne3_48

Wayne3_49

Wayne3_50
(DnaJ-like chaperonin)

Wayne3_51

Wayne3_52
(exonuclease)

Wayne3_53
(DNA recombinase)

Wayne3_54
(HNH endonuclease)

Wayne3_55
(RuvC-like resolvase)

Wayne3_56

Wayne3_57

Wayne3_58
(MazG-like nucleotide pyrophosphohydrolase)

Wayne3_59
(SSB protein)

Wayne3_60

Wayne3_61

Wayne3_62

Wayne3_63

Wayne3_64

Wayne3_65

Wayne3_66

Wayne3_67

Wayne3_68

Wayne3_69

Wayne3_70

Wayne3_71
(DNA primase/helicase)

Wayne3_72

Wayne3_73
(ribbon-helix-helix DNA binding domain protein)

Wayne3_74

Wayne3_75

Wayne3_76

Wayne3_77

Wayne3_78

Wayne3_79

Wayne3_80

Wayne3_81

Wayne3_82

Wayne3_83

Wayne3_84

Wayne3_85

Wayne3_86

Wayne3_87

Wayne3_88

Wayne3_89

Wayne3_90

Wayne3_91

Wayne3_92

Wayne3_93

Wayne3_94

Wayne3_95

Wayne3_96

Wayne3_97

Wayne3_98

Wayne3_99

Wayne3_100

Wayne3_101

Wayne3_102

Wayne3_103

Wayne3_104

Wayne3_105

Wayne3_106

Wayne3_107

Wayne3_108

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR253919
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 145
Pitt Freezer Box Grid# D2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload