| Detailed Information for Phage Wayne3 |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Diana Hoppe, Karson Gjesdal |
| Year Found | 2021 |
| Location Found | Spokane, WA United States |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 47.667432 N, 117.40321 W Map |
| Discovery Notes | This soil was collected from a mossy patch located near a curb and collected shortly after light rain. The soil was moist and soft. |
| Naming Notes | This is named after a construction worker on the Gonzaga campus who asked us about our research. We promised him we would name the phage after him. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 772 |
| Genome length (bp) | 62371 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 199 bp |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Size: .5-2mm
edges: fuzzy edges but short in width
Center Completely clear not turbid
Rings fuzzy border but no rings.
Shape: circular |
| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253919 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
145 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
