Microbacterium phage Zagie
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Detailed Information for Phage Zagie
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByAlyssa Haworth and Emma Hanson
Year Found2017
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates47.667367 N, 117.402273 W Map
Discovery NotesWe decided to use our results from our second sample: Sample 2: surfaced grass not shaded (dry, warm) Northeast of the Hughes building and on the Herak Quad.
Naming NotesOur phage's name is Zagie, which is derived from the name Magie/Maggie and it is a reference to the term "Zag" since we attend Gonzaga University.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 19, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage342
Genome length (bp)62442
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length204 bp
GC Content67.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEG
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionGonzaga University
Annotation StatusBeing Annotated (Expected completion by 6/30/2022)
Plaque NotesAfter performing our first attempt to begin forming phage-- using serial dilutions and plaque assay-- we received our first results:
We found phage within our petri dishes of 10^0, 10^-1, and one in 10^-2. Our 10^0 had phage only on half of the petri dish. Could it be because the dish was tilted to one side due to the table being slanted? The plate colors were a turbid yellow. 10^-1 had approximately 30 phage and was too tilted to one side. 10^-2 only had 2 phage, and after 10^-3, they were all solid with no sign of phage.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 65
Pitt Freezer Box Grid# G10
Available Files
Plaque PictureDownload