| Detailed Information for Phage Zagie | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Alyssa Haworth and Emma Hanson |
| Year Found | 2017 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.667367 N, 117.402273 W Map |
| Discovery Notes | We decided to use our results from our second sample: Sample 2: surfaced grass not shaded (dry, warm) Northeast of the Hughes building and on the Herak Quad. |
| Naming Notes | Our phage's name is Zagie, which is derived from the name Magie/Maggie and it is a reference to the term "Zag" since we attend Gonzaga University. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 19, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 342 |
| Genome length (bp) | 62442 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 204 bp |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Gonzaga University |
| Annotation Status | Being Annotated (Expected completion by 6/30/2022) |
| Plaque Notes | After performing our first attempt to begin forming phage-- using serial dilutions and plaque assay-- we received our first results: We found phage within our petri dishes of 10^0, 10^-1, and one in 10^-2. Our 10^0 had phage only on half of the petri dish. Could it be because the dish was tilted to one side due to the table being slanted? The plate colors were a turbid yellow. 10^-1 had approximately 30 phage and was too tilted to one side. 10^-2 only had 2 phage, and after 10^-3, they were all solid with no sign of phage. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewZagie_Draft_1 Zagie_Draft_2 Zagie_Draft_3 Zagie_Draft_4 Zagie_Draft_5 Zagie_Draft_6 Zagie_Draft_7 Zagie_Draft_8 Zagie_Draft_9 Zagie_Draft_10 Zagie_Draft_11 Zagie_Draft_12 Zagie_Draft_13 Zagie_Draft_14 Zagie_Draft_15 Zagie_Draft_16 Zagie_Draft_17 Zagie_Draft_18 Zagie_Draft_19 Zagie_Draft_20 Zagie_Draft_21 Zagie_Draft_22 Zagie_Draft_23 Zagie_Draft_24 Zagie_Draft_25 Zagie_Draft_26 Zagie_Draft_27 Zagie_Draft_28 Zagie_Draft_29 Zagie_Draft_30 Zagie_Draft_31 Zagie_Draft_32 Zagie_Draft_33 Zagie_Draft_34 Zagie_Draft_35 Zagie_Draft_36 Zagie_Draft_37 Zagie_Draft_38 Zagie_Draft_39 Zagie_Draft_40 Zagie_Draft_41 Zagie_Draft_42 Zagie_Draft_43 Zagie_Draft_44 Zagie_Draft_45 Zagie_Draft_46 Zagie_Draft_47 Zagie_Draft_48 Zagie_Draft_49 Zagie_Draft_50 Zagie_Draft_51 Zagie_Draft_52 Zagie_Draft_53 Zagie_Draft_54 Zagie_Draft_55 Zagie_Draft_56 Zagie_Draft_57 Zagie_Draft_58 Zagie_Draft_59 Zagie_Draft_60 Zagie_Draft_61 Zagie_Draft_62 Zagie_Draft_63 Zagie_Draft_64 Zagie_Draft_65 Zagie_Draft_66 Zagie_Draft_67 Zagie_Draft_68 Zagie_Draft_69 Zagie_Draft_70 Zagie_Draft_71 Zagie_Draft_72 Zagie_Draft_73 Zagie_Draft_74 Zagie_Draft_75 Zagie_Draft_76 Zagie_Draft_77 Zagie_Draft_78 Zagie_Draft_79 Zagie_Draft_80 Zagie_Draft_81 Zagie_Draft_82 Zagie_Draft_83 Zagie_Draft_84 Zagie_Draft_85 Zagie_Draft_86 Zagie_Draft_87 Zagie_Draft_88 Zagie_Draft_89 Zagie_Draft_90 Zagie_Draft_91 Zagie_Draft_92 Zagie_Draft_93 Zagie_Draft_94 Zagie_Draft_95 Zagie_Draft_96 Zagie_Draft_97 Zagie_Draft_98 Zagie_Draft_99 Zagie_Draft_100 Zagie_Draft_101 Zagie_Draft_102 Zagie_Draft_103 Zagie_Draft_104 Zagie_Draft_105 Zagie_Draft_106 Zagie_Draft_107 Zagie_Draft_108 Zagie_Draft_109 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 65 |
| Pitt Freezer Box Grid# | G10 |
| Available Files | |
| Plaque Picture | Download |