| Detailed Information for Phage BabyDotz | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Ethan Dotzler, Jonathan Nyandu Kanyinda |
| Year Found | 2020 |
| Location Found | Moorhead, MN Clay |
| Finding Institution | Minnesota State University Moorhead |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -2°C |
| GPS Coordinates | 46.8673 N, 96.762 W Map |
| Discovery Notes | This sample was collected on January 27th, 2020. The conditions of the day were cold, with a temperature of 28*F. This sample was collected between two Pine trees out on the Minnesota State University Moorhead Campus Mall, with the sample being comprised of dirt that was collected from approximately 1 inch below the visible surface. |
| Naming Notes | We gratefully dedicate the name of our phage to the wonderful discoverer, Ethan Dotzler. This is the first of two samples found in relatively the same area, and it is now the youngest member of the Dotzler Family. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1387 |
| Genome length (bp) | 63076 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 217 bp |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAltheas AluminumJesus BabyDotz BirdInFrench Blab CandC Casend DonaldDuck EverythinBagel Fizzles FrankDeliGuy Fransoyer Fregley Gazebo Grassboy Hyperion Jehoshaphat Judebell Kelcole KillerQueen Kyva Llemily Lonelysoil Marcie Mashley Mentos MorillMayhem Namago Nike OneinaGillian Pepe25 Phabia Phinky Quammi RobinRose Romm Rowlf RubyRalph Rudy SadLad SallyK Sillytadpoles Squash Statler StrawberryJamm Teehee Tempo Tissue Viceroy Wayne3 Wheelie Wilca Zagie Zhafia |
| Annotating Institution | Xavier University of Louisiana |
| Annotation Status | In GenBank |
| Plaque Notes | It was one of a few visible plaques on a series of plates, and was further isolated after collecting samples from those plaques. The Plaques were clear, and were relatively round in shape, somewhat in-between the shapes of a circle and an oval, but not quite either of the two. After further investigation, and thanks to the TEM photos received, we were able to identify this phage as a Siphoviridae. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBabyDotz_1 BabyDotz_2 BabyDotz_3 BabyDotz_4 BabyDotz_5 BabyDotz_6 BabyDotz_7 BabyDotz_8 BabyDotz_9 BabyDotz_10 BabyDotz_11 BabyDotz_12 BabyDotz_13 BabyDotz_14 BabyDotz_15 BabyDotz_16 BabyDotz_17 BabyDotz_18 BabyDotz_19 BabyDotz_20 BabyDotz_21 BabyDotz_22 BabyDotz_23 BabyDotz_24 BabyDotz_25 BabyDotz_26 BabyDotz_27 BabyDotz_28 BabyDotz_29 BabyDotz_30 BabyDotz_31 BabyDotz_32 BabyDotz_33 BabyDotz_34 BabyDotz_35 BabyDotz_36 BabyDotz_37 BabyDotz_38 BabyDotz_39 BabyDotz_40 BabyDotz_41 BabyDotz_42 BabyDotz_43 BabyDotz_44 BabyDotz_45 BabyDotz_46 BabyDotz_47 BabyDotz_48 BabyDotz_49 BabyDotz_50 BabyDotz_51 BabyDotz_52 BabyDotz_53 BabyDotz_54 BabyDotz_55 BabyDotz_56 BabyDotz_57 BabyDotz_58 BabyDotz_59 BabyDotz_60 BabyDotz_61 BabyDotz_62 BabyDotz_63 BabyDotz_64 BabyDotz_65 BabyDotz_66 BabyDotz_67 BabyDotz_68 BabyDotz_69 BabyDotz_70 BabyDotz_71 BabyDotz_72 BabyDotz_73 BabyDotz_74 BabyDotz_75 BabyDotz_76 BabyDotz_77 BabyDotz_78 BabyDotz_79 BabyDotz_80 BabyDotz_81 BabyDotz_82 BabyDotz_83 BabyDotz_84 BabyDotz_85 BabyDotz_86 BabyDotz_87 BabyDotz_88 BabyDotz_89 BabyDotz_90 BabyDotz_91 BabyDotz_92 BabyDotz_93 BabyDotz_94 BabyDotz_95 BabyDotz_96 BabyDotz_97 BabyDotz_98 BabyDotz_99 BabyDotz_100 BabyDotz_101 BabyDotz_102 BabyDotz_103 BabyDotz_104 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR613478 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 6.67 X 10^10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |