| Detailed Information for Phage BabyDotz | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Ethan Dotzler, Jonathan Nyandu Kanyinda |
| Year Found | 2020 |
| Location Found | Moorhead, MN Clay |
| Finding Institution | Minnesota State University Moorhead |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -2°C |
| GPS Coordinates | 46.8673 N, 96.762 W Map |
| Discovery Notes | This sample was collected on January 27th, 2020. The conditions of the day were cold, with a temperature of 28*F. This sample was collected between two Pine trees out on the Minnesota State University Moorhead Campus Mall, with the sample being comprised of dirt that was collected from approximately 1 inch below the visible surface. |
| Naming Notes | We gratefully dedicate the name of our phage to the wonderful discoverer, Ethan Dotzler. This is the first of two samples found in relatively the same area, and it is now the youngest member of the Dotzler Family. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1387 |
| Genome length (bp) | 63076 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 217 bp |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Minnesota State University Moorhead |
| Annotation Status | Overdue (Expected completion by 5/20/2022) |
| Plaque Notes | It was one of a few visible plaques on a series of plates, and was further isolated after collecting samples from those plaques. The Plaques were clear, and were relatively round in shape, somewhat in-between the shapes of a circle and an oval, but not quite either of the two. After further investigation, and thanks to the TEM photos received, we were able to identify this phage as a Siphoviridae. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBabyDotz_Draft_1 BabyDotz_Draft_2 BabyDotz_Draft_3 BabyDotz_Draft_4 BabyDotz_Draft_5 BabyDotz_Draft_6 BabyDotz_Draft_7 BabyDotz_Draft_8 BabyDotz_Draft_9 BabyDotz_Draft_10 BabyDotz_Draft_11 BabyDotz_Draft_12 BabyDotz_Draft_13 BabyDotz_Draft_14 BabyDotz_Draft_15 BabyDotz_Draft_16 BabyDotz_Draft_17 BabyDotz_Draft_18 BabyDotz_Draft_19 BabyDotz_Draft_20 BabyDotz_Draft_21 BabyDotz_Draft_22 BabyDotz_Draft_23 BabyDotz_Draft_24 BabyDotz_Draft_25 BabyDotz_Draft_26 BabyDotz_Draft_27 BabyDotz_Draft_28 BabyDotz_Draft_29 BabyDotz_Draft_30 BabyDotz_Draft_31 BabyDotz_Draft_32 BabyDotz_Draft_33 BabyDotz_Draft_34 BabyDotz_Draft_35 BabyDotz_Draft_36 BabyDotz_Draft_37 BabyDotz_Draft_38 BabyDotz_Draft_39 BabyDotz_Draft_40 BabyDotz_Draft_41 BabyDotz_Draft_42 BabyDotz_Draft_43 BabyDotz_Draft_44 BabyDotz_Draft_45 BabyDotz_Draft_46 BabyDotz_Draft_47 BabyDotz_Draft_48 BabyDotz_Draft_49 BabyDotz_Draft_50 BabyDotz_Draft_51 BabyDotz_Draft_52 BabyDotz_Draft_53 BabyDotz_Draft_54 BabyDotz_Draft_55 BabyDotz_Draft_56 BabyDotz_Draft_57 BabyDotz_Draft_58 BabyDotz_Draft_59 BabyDotz_Draft_60 BabyDotz_Draft_61 BabyDotz_Draft_62 BabyDotz_Draft_63 BabyDotz_Draft_64 BabyDotz_Draft_65 BabyDotz_Draft_66 BabyDotz_Draft_67 BabyDotz_Draft_68 BabyDotz_Draft_69 BabyDotz_Draft_70 BabyDotz_Draft_71 BabyDotz_Draft_72 BabyDotz_Draft_73 BabyDotz_Draft_74 BabyDotz_Draft_75 BabyDotz_Draft_76 BabyDotz_Draft_77 BabyDotz_Draft_78 BabyDotz_Draft_79 BabyDotz_Draft_80 BabyDotz_Draft_81 BabyDotz_Draft_82 BabyDotz_Draft_83 BabyDotz_Draft_84 BabyDotz_Draft_85 BabyDotz_Draft_86 BabyDotz_Draft_87 BabyDotz_Draft_88 BabyDotz_Draft_89 BabyDotz_Draft_90 BabyDotz_Draft_91 BabyDotz_Draft_92 BabyDotz_Draft_93 BabyDotz_Draft_94 BabyDotz_Draft_95 BabyDotz_Draft_96 BabyDotz_Draft_97 BabyDotz_Draft_98 BabyDotz_Draft_99 BabyDotz_Draft_100 BabyDotz_Draft_101 BabyDotz_Draft_102 BabyDotz_Draft_103 BabyDotz_Draft_104 BabyDotz_Draft_105 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 6.67 X 10^10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |