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Microbacterium phage RubyRalph
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Detailed Information for Phage RubyRalph
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Found ByLexia Sakowski and Sena Sperloen
Year Found2018
Location FoundRiver Falls, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates44.852 N, 92.6187 W Map
Discovery NotesSoil sample from Sena Sperloen
Soil was collected on 9/9/2018 at 7:37pm. It was collected on a trail by the river/bridge by central building. The soil was loose, slightly moist, but mostly dry. It was dugged up 3.5 inches and 65 degrees F. It was cloudy and cool with lots of vegetation around.
Naming NotesIt is named after my dog, Ruby.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 19, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage144
Genome length (bp)61880
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length209 bp
GC Content69.0%
Sequencing NotesThis phage was sequenced as part of a DOGEMS sample, then the sequence was matched to the lysate by PCR.
Fasta file available?Yes: Download fasta file
Characterization
ClusterEG
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 10/19/2019)
Plaque NotesThe size of the phage is extremely tiny. They were clear with no halo. It was uniformed in size.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 88
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload