Microbacterium phage RubyRalph
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Detailed Information for Phage RubyRalph
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Found ByLexia Sakowski and Sena Sperloen
Year Found2018
Location FoundRiver Falls, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates44.852 N, 92.6187 W Map
Discovery NotesSoil sample from Sena Sperloen
Soil was collected on 9/9/2018 at 7:37pm. It was collected on a trail by the river/bridge by central building. The soil was loose, slightly moist, but mostly dry. It was dugged up 3.5 inches and 65 degrees F. It was cloudy and cool with lots of vegetation around.
Naming NotesIt is named after my dog, Ruby.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 19, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage144
Genome length (bp)61880
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length209 bp
GC Content69.0%
Sequencing NotesThis phage was sequenced as part of a DOGEMS sample, then the sequence was matched to the lysate by PCR.
Fasta file available?Yes: Download fasta file
Characterization
ClusterEG
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque NotesThe size of the phage is extremely tiny. They were clear with no halo. It was uniformed in size.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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RubyRalph_1

RubyRalph_2

RubyRalph_3
(ParB-like nuclease domain protein)

RubyRalph_4

RubyRalph_5

RubyRalph_6

RubyRalph_7

RubyRalph_8

RubyRalph_9

RubyRalph_10
(DprA-like DNA processing chain A)

RubyRalph_11

RubyRalph_12
(helix-turn-helix DNA binding domain protein)

RubyRalph_13
(helix-turn-helix DNA binding domain protein)

RubyRalph_14

RubyRalph_15

RubyRalph_16
(ribbon-helix-helix DNA binding domain protein)

RubyRalph_17

RubyRalph_18
(helix-turn-helix DNA binding domain protein)

RubyRalph_19

RubyRalph_20
(ribbon-helix-helix DNA binding domain protein)

RubyRalph_21
(terminase)

RubyRalph_22

RubyRalph_23

RubyRalph_24
(portal protein)

RubyRalph_25
(capsid maturation protease)

RubyRalph_26

RubyRalph_27
(major capsid protein)

RubyRalph_28

RubyRalph_29

RubyRalph_30
(major tail protein)

RubyRalph_31

RubyRalph_32
(phosphoesterase)

RubyRalph_33
(head-to-tail adaptor)

RubyRalph_34
(minor capsid protein)

RubyRalph_35

RubyRalph_36
(tail assembly chaperone)

RubyRalph_37
(tail assembly chaperone)

RubyRalph_38
(tape measure protein)

RubyRalph_39
(minor tail protein)

RubyRalph_40
(minor tail protein)

RubyRalph_41
(minor tail protein)

RubyRalph_42
(minor tail protein)

RubyRalph_43

RubyRalph_44

RubyRalph_45

RubyRalph_46
(lysin A)

RubyRalph_47

RubyRalph_48

RubyRalph_49

RubyRalph_50

RubyRalph_51
(DnaJ-like chaperonin)

RubyRalph_52

RubyRalph_53
(MazG-like nucleotide pyrophosphohydrolase)

RubyRalph_54
(exonuclease)

RubyRalph_55

RubyRalph_56
(DNA recombinase)

RubyRalph_57
(HNH endonuclease)

RubyRalph_58

RubyRalph_59
(RuvC-like resolvase)

RubyRalph_60

RubyRalph_61
(ssDNA binding protein)

RubyRalph_62

RubyRalph_63

RubyRalph_64

RubyRalph_65

RubyRalph_66

RubyRalph_67

RubyRalph_68
(helix-turn-helix DNA binding domain protein)

RubyRalph_69
(helix-turn-helix DNA binding domain protein)

RubyRalph_70
(DNA primase)

RubyRalph_71

RubyRalph_72

RubyRalph_73

RubyRalph_74

RubyRalph_75

RubyRalph_76

RubyRalph_77

RubyRalph_78
(MazG-like nucleotide pyrophosphohydrolase)

RubyRalph_79

RubyRalph_80

RubyRalph_81

RubyRalph_82

RubyRalph_83

RubyRalph_84

RubyRalph_85

RubyRalph_86

RubyRalph_87

RubyRalph_88

RubyRalph_89

RubyRalph_90

RubyRalph_91

RubyRalph_92

RubyRalph_93

RubyRalph_94

RubyRalph_95

RubyRalph_96

RubyRalph_97

RubyRalph_98

RubyRalph_99

RubyRalph_100

RubyRalph_101

RubyRalph_102

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN908694
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 88
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload