| Detailed Information for Phage Bombitas |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lidia Peralta |
| Year Found | 2022 |
| Location Found | Rock Hill, SC United States |
| Finding Institution | Winthrop University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.941325 N, 81.034067 W Map |
| Discovery Notes | Collected under first small tree to the left. Area under tree had bed. When digging, found a lot of grubs, and there were a lot of roots. Many hornets and earwigs were also in the surrounding area. Soil sample had red clay in it and a good bit of organic matter and particles in it. For weather, it was foggy and cloudy, temperature was 22 degrees centigrade. |
| Naming Notes | Bombitas is a nickname my father gave me when I was a baby. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 327 |
| Genome length (bp) | 110129 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Winthrop University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques tend to be small, about one millimeter in size. Plaques are numerous on plates, often clearing entire plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 226 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR195048 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
153 |
| Pitt Freezer Box Grid# |
A5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
