Mycobacterium phage Bombitas
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Detailed Information for Phage Bombitas
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLidia Peralta
Year Found2022
Location FoundRock Hill, SC United States
Finding InstitutionWinthrop University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates34.941325 N, 81.034067 W Map
Discovery NotesCollected under first small tree to the left. Area under tree had bed. When digging, found a lot of grubs, and there were a lot of roots. Many hornets and earwigs were also in the surrounding area. Soil sample had red clay in it and a good bit of organic matter and particles in it. For weather, it was foggy and cloudy, temperature was 22 degrees centigrade.
Naming NotesBombitas is a nickname my father gave me when I was a baby.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 14, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage327
Genome length (bp)110129
Character of genome ends3' Sticky Overhang
Overhang Length4 bases
Overhang SequenceATCC
GC Content60.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterJ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWinthrop University
Annotation StatusIn GenBank
Plaque NotesPlaques tend to be small, about one millimeter in size. Plaques are numerous on plates, often clearing entire plates.
MorphotypeSiphoviridae
Number of Genes226
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Bombitas_1
(terminase)

Bombitas_2

Bombitas_3

Bombitas_4

Bombitas_5

Bombitas_6

Bombitas_7
(terminase large subunit)

Bombitas_8

Bombitas_9
(portal protein)

Bombitas_10
(capsid maturation protease)

Bombitas_11
(major capsid protein)

Bombitas_12
(glycosyltransferase)

Bombitas_13
(serine/threonine kinase)

Bombitas_14
(methyltransferase)

Bombitas_15
(glycosyltransferase)

Bombitas_16
(glycosyltransferase)

Bombitas_17
(glycosyltransferase)

Bombitas_18
(peptidase)

Bombitas_19
(UDP-glucose dehydrogenase)

Bombitas_20
(glycosyltransferase)

Bombitas_21

Bombitas_22
(head-to-tail adaptor)

Bombitas_23
(head-to-tail stopper)

Bombitas_24

Bombitas_25
(tail terminator)

Bombitas_26

Bombitas_27

Bombitas_28
(major tail protein)

Bombitas_29
(tail assembly chaperone)

Bombitas_30
(tail assembly chaperone)

Bombitas_31
(tape measure protein)

Bombitas_32
(minor tail protein)

Bombitas_33
(minor tail protein)

Bombitas_34
(minor tail protein)

Bombitas_35
(minor tail protein)

Bombitas_36
(minor tail protein)

Bombitas_37

Bombitas_38

Bombitas_39

Bombitas_40

Bombitas_41

Bombitas_42

Bombitas_43
(lysin A)

Bombitas_44

Bombitas_45
(HNH endonuclease)

Bombitas_46
(lysin B)

Bombitas_47
(holin)

Bombitas_48

Bombitas_49
(thioredoxin)

Bombitas_50

Bombitas_51

Bombitas_52

Bombitas_53

Bombitas_54

Bombitas_55

Bombitas_56
(Lsr2-like DNA bridging protein)

Bombitas_57

Bombitas_58

Bombitas_59

Bombitas_60

Bombitas_61

Bombitas_62

Bombitas_63

Bombitas_64

Bombitas_65

Bombitas_66

Bombitas_67

Bombitas_68

Bombitas_69

Bombitas_70
(excise)

Bombitas_71
(tyrosine integrase)

Bombitas_72

Bombitas_73

Bombitas_74

Bombitas_75

Bombitas_76

Bombitas_77

Bombitas_78

Bombitas_79

Bombitas_80

Bombitas_81

Bombitas_82

Bombitas_83

Bombitas_84

Bombitas_85
(immunity repressor)

Bombitas_86
(helix-turn-helix DNA binding domain protein)

Bombitas_87

Bombitas_88

Bombitas_89
(esterase)

Bombitas_90

Bombitas_91

Bombitas_92

Bombitas_93

Bombitas_94

Bombitas_95
(HNH endonuclease)

Bombitas_96

Bombitas_97

Bombitas_98

Bombitas_99

Bombitas_100

Bombitas_101

Bombitas_102

Bombitas_103

Bombitas_104

Bombitas_105
(RecB-like exonuclease/helicase)

Bombitas_106

Bombitas_107

Bombitas_108

Bombitas_109
(DNA methyltransferase)

Bombitas_110
(DNA methyltransferase)

Bombitas_111

Bombitas_112

Bombitas_113
(tRNA nucleotidyltransferase)

Bombitas_114

Bombitas_115

Bombitas_116
(polynucleotide kinase)

Bombitas_117

Bombitas_118
(endonuclease VII)

Bombitas_119
(WhiB family transcription factor)

Bombitas_120
(helix-turn-helix DNA binding domain protein)

Bombitas_121

Bombitas_122

Bombitas_123

Bombitas_124

Bombitas_125
(DNA polymerase/primase)

Bombitas_126

Bombitas_127

Bombitas_128

Bombitas_129

Bombitas_130

Bombitas_131

Bombitas_132
(helix-turn-helix DNA binding domain protein)

Bombitas_133

Bombitas_134

Bombitas_135

Bombitas_136
(DNA primase/helicase)

Bombitas_137

Bombitas_138

Bombitas_139

Bombitas_140
(HNH endonuclease)

Bombitas_141

Bombitas_142

Bombitas_143

Bombitas_144
(RNA ligase)

Bombitas_145
(Clp-like protease)

Bombitas_146
(DnaQ-like DNA polymerase III subunit)

Bombitas_147

Bombitas_148

Bombitas_149

Bombitas_150

Bombitas_151
(MazG-like nucleotide pyrophosphohydrolase)

Bombitas_152

Bombitas_153
(helix-turn-helix DNA binding domain protein)

Bombitas_154
(metallophosphatase)

Bombitas_155

Bombitas_156

Bombitas_157

Bombitas_158

Bombitas_159

Bombitas_160

Bombitas_161

Bombitas_162
(metallophosphoesterase)

Bombitas_163

Bombitas_164

Bombitas_165

Bombitas_166

Bombitas_167

Bombitas_168

Bombitas_169 — tRNA
(tRNA-Gly(tcc))

Bombitas_170

Bombitas_171

Bombitas_172

Bombitas_173

Bombitas_174

Bombitas_175

Bombitas_176

Bombitas_177
(helix-turn-helix DNA binding domain protein)

Bombitas_178

Bombitas_179

Bombitas_180

Bombitas_181
(Imm-like superinfection immunity protein)

Bombitas_182

Bombitas_183

Bombitas_184
(FtsK-like DNA translocase)

Bombitas_185

Bombitas_186

Bombitas_187

Bombitas_188
(Ku-like dsDNA break-binding protein)

Bombitas_189

Bombitas_190

Bombitas_191

Bombitas_192

Bombitas_193

Bombitas_194

Bombitas_195

Bombitas_196

Bombitas_197

Bombitas_198

Bombitas_199

Bombitas_200

Bombitas_201

Bombitas_202

Bombitas_203

Bombitas_204

Bombitas_205

Bombitas_206

Bombitas_207

Bombitas_208

Bombitas_209

Bombitas_210

Bombitas_211

Bombitas_212

Bombitas_213

Bombitas_214

Bombitas_215

Bombitas_216

Bombitas_217

Bombitas_218

Bombitas_219

Bombitas_220

Bombitas_221

Bombitas_222

Bombitas_223

Bombitas_224

Bombitas_225

Bombitas_226

Bombitas_227

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR195048
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 153
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload