| Detailed Information for Phage Cashington | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Molly Fuchs Townzen, Jillian Hines |
| Year Found | 2023 |
| Location Found | Spokane, WA United States of America |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.66637 N, 117.40154 W Map |
| Discovery Notes | Found behind Gonzaga's library under a tree in winter. Soil was firm due to the the cold January temperatures. The weather upon discovery was 38-32 degrees Fahrenheit. The soil was packed tight and was silt-like. We also dug out our sample from under a bed of grass so we anticipated some organic material to be in our soil. |
| Naming Notes | Cashington is a combination of California and Washington - the two states we are from. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 30, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1764 |
| Genome length (bp) | 41600 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAbigail Albedo Albright AnnaLie Armstrong Arroyo AvGardian Avocadoman BabyDaisy BabyYoda Bachaco BAjuniper BelmontSKP Bernstein Brahms BubbaBear Buldak Burritobowl Cashington Celaena ChiliPepper Clayda5 CliffHanger Coltrane CroZenni DickRichards Didgeridoo DirtyBubble Dismas Doobus Eden Elva Eula Finalfrontier FlameThrower Franklin22 Gack Icarian IndyLu Johnathan Jovita Kate33 Katzastrophic Kenzers Kieran Lahqtemish LimaBean Loviatar Lynlen Nicky22 Olliecat Phisb QMacho Quenya Rollins Rona SanaSana SansAfet SarBear Sharkboy Shayna Skylord Slay Squircle Stoor Stromboli SunnyD Swervy Teagster TukTuk Vitas WalkingDead |
| Annotating Institution | Gonzaga University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Cashington’s plaque morphology includes small plaques roughly ~ 2 mm in diameter, and the plaques are slightly cloudy with fuzzy edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewCashington_1 Cashington_2 Cashington_3 Cashington_4 Cashington_5 Cashington_6 Cashington_7 Cashington_8 Cashington_9 Cashington_10 Cashington_11 Cashington_12 Cashington_13 Cashington_14 Cashington_15 Cashington_16 Cashington_17 Cashington_18 Cashington_19 Cashington_20 Cashington_21 Cashington_22 Cashington_23 Cashington_24 Cashington_25 Cashington_26 Cashington_27 Cashington_28 Cashington_29 Cashington_30 Cashington_31 Cashington_32 Cashington_33 Cashington_34 Cashington_35 Cashington_36 Cashington_37 Cashington_38 Cashington_39 Cashington_40 Cashington_41 Cashington_42 Cashington_43 Cashington_44 Cashington_45 Cashington_46 Cashington_47 Cashington_48 Cashington_49 Cashington_50 Cashington_51 Cashington_52 Cashington_53 Cashington_54 Cashington_55 Cashington_56 Cashington_57 Cashington_58 Cashington_59 Cashington_60 Cashington_61 Cashington_62 Cashington_63 Cashington_64 Cashington_65 Cashington_66 Cashington_67 Cashington_68 — tRNA Cashington_69 Cashington_70 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 189 |
| Pitt Freezer Box Grid# | D7 |