Streptomyces phage Chymera
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Detailed Information for Phage Chymera
Discovery Information
Isolation HostStreptomyces venezuelae ATCC 10712
Former namesChimera
Found ByConner Kennedy and Stetson Norried
Year Found2014
Location FoundSanger, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.354419 N, 97.087331 W Map
Discovery NotesChymera was isolated from a soil sample collected from Lake Ray Roberts in Sanger, Texas in late August. It was taken roughly 3 meters away from a stagnant pond located a couple hundred meters from the lake itself. It was collected a foot deep in moist ground, mid-day. The temperature of the day was in the upper seventies.
Naming NotesNamed for the mythological Greek monster, the Chimera. Notable for its maliciousness, odd properties, and tendency to annihilate agar plates.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage201
Sanger Finishing Reactions2
Genome length (bp)34742
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGCGGGGGGG
GC Content71.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesIncredibly small plaques, that grow very rapidly. Has the capability to completely lyse an agar plate in 24 hours at a medium titer. They are clear, and form near-perfect circles.
MorphotypeSiphoviridae
Number of Genes55
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU958700
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 36
Pitt Freezer Box Grid# A7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload